Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles
Sophisticated genetic tools to modify essential biological processes at the molecular level are pivotal in elucidating the molecular pathogenesis of Clostridium difficile, a major cause of healthcare associated disease. Here we have developed an efficient procedure for making precise alterations to...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Published: |
Public Library of Science
2013
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| Online Access: | https://eprints.nottingham.ac.uk/2536/ |