Cloning the enterotoxin gene from Clostridium perfringens type A
A C. perfringens type A genomic library was constructed in E. coli by banking overlapping 6-10 kbp Hind III fragments of chromosomal DNA from the enterotoxin (CPE) positive strain NCTC 8239 into the pUC derived vector pHG165. The library was screened by colony hybridization with a degenerate 26 bp o...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
| Published: |
1991
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| Online Access: | https://eprints.nottingham.ac.uk/12195/ |