Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics

Fruit of salak (Salacca zalacca) is tradition ally used and commercialized as an an tidiabetic agent. How ever, the scientific evidence to prove this traditional use is lack ing. This re search was aimed to evaluate the metabolic changes of obese-diabetic (OBDC) rats treated with S. zalacca fruit...

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Main Authors: Saleh, Mohammed S M, Siddiqui, Mohammad Jamshed Ahmad, Mediani, Ahmed, Ahmed, Qamar Uddin, Mat So'ad, Siti Zaiton, Saidi-Besbes, Salima, Elnaem, Mohamed Hassan Abdelaziz, Othman, Hairol Azrin, Ismail, Nor Hadiani
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Language:English
English
English
English
Published: Elseviers 2020
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Online Access:http://irep.iium.edu.my/81676/
http://irep.iium.edu.my/81676/7/evidence%20email-jamshed.pdf
http://irep.iium.edu.my/81676/13/1-s2.0-S096399692030572X-main.pdf
http://irep.iium.edu.my/81676/19/81676_Modulation%20of%20metabolic%20alterations%20of%20obese_scopus.pdf
http://irep.iium.edu.my/81676/25/81676_Modulation%20of%20metabolic%20alterations%20of%20obese%20diabetic%20rats_WoS.pdf
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author Saleh, Mohammed S M
Siddiqui, Mohammad Jamshed Ahmad
Mediani, Ahmed
Ahmed, Qamar Uddin
Mat So'ad, Siti Zaiton
Saidi-Besbes, Salima
Elnaem, Mohamed Hassan Abdelaziz
Othman, Hairol Azrin
Ismail, Nor Hadiani
author_facet Saleh, Mohammed S M
Siddiqui, Mohammad Jamshed Ahmad
Mediani, Ahmed
Ahmed, Qamar Uddin
Mat So'ad, Siti Zaiton
Saidi-Besbes, Salima
Elnaem, Mohamed Hassan Abdelaziz
Othman, Hairol Azrin
Ismail, Nor Hadiani
author_sort Saleh, Mohammed S M
building IIUM Repository
collection Online Access
description Fruit of salak (Salacca zalacca) is tradition ally used and commercialized as an an tidiabetic agent. How ever, the scientific evidence to prove this traditional use is lack ing. This re search was aimed to evaluate the metabolic changes of obese-diabetic (OBDC) rats treated with S. zalacca fruit using proton-nuclear magnetic resonance ( H NMR)-based metabolomics approach. This re search presents the first report on the in vitro antidiabetic effect of S. zalacca fruits ex tract us ing this approach. The obtained results in dicated that the administration of 400 mg/ kg bw of 60% ethanolic S. zalacca extract for 6 weeks significantly de creased the blood glucose level and normalized the blood lipid pro file of the OBDC rats. The potential biomarkers in urine were 2-oxoglutarate, alanine, leucine, succinate 3-hydroxy butyrate, taurine, betaine, allantoin, acetate, dimethylamine, creatine, creatinine, glucose, phenyl-acetyl glycine, and hippurate. Based on the data obtained, the metabolite pro files of the urine of treated rats by the 60% ethanolic extract could not be fully improved the metabolic complications of diabetic rats. The ex tract of S. zalacca fruit was able to de crease the ketones bodies as 3-hydroxy butyrate and acetoacetate. It has also improved energy metabolism, involving glucose, acetate, lactate, 2-hydroxy butyrate, 2-oxoglutarate, citrate, and succinate. More over, it decreased metabolites from gut microflora, including choline. This extract had significant effect on amino acid metabolism, metabolites from gut microflora, bile acid metabolism and creatine. The result can further support the traditional claims of S. zalacca fruits in management of diabetes. This finding might bevaluable in understanding the molecular mechanism and pharmacological properties of this medicinal plant for managing diabetes mellitus.
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institution_category Local University
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publishDate 2020
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spelling iium-816762022-07-15T02:18:51Z http://irep.iium.edu.my/81676/ Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics Saleh, Mohammed S M Siddiqui, Mohammad Jamshed Ahmad Mediani, Ahmed Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Saidi-Besbes, Salima Elnaem, Mohamed Hassan Abdelaziz Othman, Hairol Azrin Ismail, Nor Hadiani QD Chemistry Fruit of salak (Salacca zalacca) is tradition ally used and commercialized as an an tidiabetic agent. How ever, the scientific evidence to prove this traditional use is lack ing. This re search was aimed to evaluate the metabolic changes of obese-diabetic (OBDC) rats treated with S. zalacca fruit using proton-nuclear magnetic resonance ( H NMR)-based metabolomics approach. This re search presents the first report on the in vitro antidiabetic effect of S. zalacca fruits ex tract us ing this approach. The obtained results in dicated that the administration of 400 mg/ kg bw of 60% ethanolic S. zalacca extract for 6 weeks significantly de creased the blood glucose level and normalized the blood lipid pro file of the OBDC rats. The potential biomarkers in urine were 2-oxoglutarate, alanine, leucine, succinate 3-hydroxy butyrate, taurine, betaine, allantoin, acetate, dimethylamine, creatine, creatinine, glucose, phenyl-acetyl glycine, and hippurate. Based on the data obtained, the metabolite pro files of the urine of treated rats by the 60% ethanolic extract could not be fully improved the metabolic complications of diabetic rats. The ex tract of S. zalacca fruit was able to de crease the ketones bodies as 3-hydroxy butyrate and acetoacetate. It has also improved energy metabolism, involving glucose, acetate, lactate, 2-hydroxy butyrate, 2-oxoglutarate, citrate, and succinate. More over, it decreased metabolites from gut microflora, including choline. This extract had significant effect on amino acid metabolism, metabolites from gut microflora, bile acid metabolism and creatine. The result can further support the traditional claims of S. zalacca fruits in management of diabetes. This finding might bevaluable in understanding the molecular mechanism and pharmacological properties of this medicinal plant for managing diabetes mellitus. Elseviers 2020-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/81676/7/evidence%20email-jamshed.pdf application/pdf en http://irep.iium.edu.my/81676/13/1-s2.0-S096399692030572X-main.pdf application/pdf en http://irep.iium.edu.my/81676/19/81676_Modulation%20of%20metabolic%20alterations%20of%20obese_scopus.pdf application/pdf en http://irep.iium.edu.my/81676/25/81676_Modulation%20of%20metabolic%20alterations%20of%20obese%20diabetic%20rats_WoS.pdf Saleh, Mohammed S M and Siddiqui, Mohammad Jamshed Ahmad and Mediani, Ahmed and Ahmed, Qamar Uddin and Mat So'ad, Siti Zaiton and Saidi-Besbes, Salima and Elnaem, Mohamed Hassan Abdelaziz and Othman, Hairol Azrin and Ismail, Nor Hadiani (2020) Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics. Food Research International, 137. ISSN 0963-9969 https://www.sciencedirect.com/science/article/abs/pii/S096399692030572X?via%3Dihub 10.1016/j.foodres.2020.109547
spellingShingle QD Chemistry
Saleh, Mohammed S M
Siddiqui, Mohammad Jamshed Ahmad
Mediani, Ahmed
Ahmed, Qamar Uddin
Mat So'ad, Siti Zaiton
Saidi-Besbes, Salima
Elnaem, Mohamed Hassan Abdelaziz
Othman, Hairol Azrin
Ismail, Nor Hadiani
Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics
title Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics
title_full Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics
title_fullStr Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics
title_full_unstemmed Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics
title_short Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics
title_sort modulation of metabolic alterations of obese diabetic rats upon treatment with salacca zalacca fruits extract using h nmr-based metabolomics
topic QD Chemistry
url http://irep.iium.edu.my/81676/
http://irep.iium.edu.my/81676/
http://irep.iium.edu.my/81676/
http://irep.iium.edu.my/81676/7/evidence%20email-jamshed.pdf
http://irep.iium.edu.my/81676/13/1-s2.0-S096399692030572X-main.pdf
http://irep.iium.edu.my/81676/19/81676_Modulation%20of%20metabolic%20alterations%20of%20obese_scopus.pdf
http://irep.iium.edu.my/81676/25/81676_Modulation%20of%20metabolic%20alterations%20of%20obese%20diabetic%20rats_WoS.pdf