Study of cells attachment using impedance spectroscopy technique

In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell...

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Main Authors:	Nordin, Anis Nurashikin, S.M., Arifuzzaman, Mel, Maizirwan, Spray, David, Voiculescu, Ioana
Format:	Proceeding Paper
Language:	English
Published:	2011
Subjects:	TA164 Bioengineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/8147/ http://irep.iium.edu.my/8147/1/IMECE2011-62919.pdf

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author	Nordin, Anis Nurashikin S.M., Arifuzzaman Mel, Maizirwan Spray, David Voiculescu, Ioana
author_facet	Nordin, Anis Nurashikin S.M., Arifuzzaman Mel, Maizirwan Spray, David Voiculescu, Ioana
author_sort	Nordin, Anis Nurashikin
building	IIUM Repository
collection	Online Access
description	In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL Multiphysics, a commercially available modeling package.
first_indexed	2025-11-14T14:38:25Z
format	Proceeding Paper
id	iium-8147
institution	International Islamic University Malaysia
institution_category	Local University
language	English
last_indexed	2025-11-14T14:38:25Z
publishDate	2011
recordtype	eprints
repository_type	Digital Repository
spelling	iium-81472011-11-29T07:51:07Z http://irep.iium.edu.my/8147/ Study of cells attachment using impedance spectroscopy technique Nordin, Anis Nurashikin S.M., Arifuzzaman Mel, Maizirwan Spray, David Voiculescu, Ioana TA164 Bioengineering TP248.13 Biotechnology In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL Multiphysics, a commercially available modeling package. 2011-11 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/8147/1/IMECE2011-62919.pdf Nordin, Anis Nurashikin and S.M., Arifuzzaman and Mel, Maizirwan and Spray, David and Voiculescu, Ioana (2011) Study of cells attachment using impedance spectroscopy technique. In: International Mechanical Engineering Congress & Exposition (IMECE 2011), 11-17 November 2011, Denver, Colorado. http://www.asmeconferences.org/Congress2011/
spellingShingle	TA164 Bioengineering TP248.13 Biotechnology Nordin, Anis Nurashikin S.M., Arifuzzaman Mel, Maizirwan Spray, David Voiculescu, Ioana Study of cells attachment using impedance spectroscopy technique
title	Study of cells attachment using impedance spectroscopy technique
title_full	Study of cells attachment using impedance spectroscopy technique
title_fullStr	Study of cells attachment using impedance spectroscopy technique
title_full_unstemmed	Study of cells attachment using impedance spectroscopy technique
title_short	Study of cells attachment using impedance spectroscopy technique
title_sort	study of cells attachment using impedance spectroscopy technique
topic	TA164 Bioengineering TP248.13 Biotechnology
url	http://irep.iium.edu.my/8147/ http://irep.iium.edu.my/8147/ http://irep.iium.edu.my/8147/1/IMECE2011-62919.pdf

Study of cells attachment using impedance spectroscopy technique

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