Microbial hydrolytic enzymes: In silico studies between polar and tropical regions
Enzyme is important as biocatalyst for industrial and biotechnological applications. Cold active enzymes have showed many advantages compare to mesophilic enzymes. Their cold active and thermolabile characteristics have shown potential benefits in many industries. In silico characterization of hyd...
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| Language: | English English English |
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Elsevier
2019
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| Online Access: | http://irep.iium.edu.my/74627/ http://irep.iium.edu.my/74627/7/74627%20Microbial%20hydrolytic%20enzymes.pdf http://irep.iium.edu.my/74627/8/74627%20Microbial%20hydrolytic%20enzymes%20SCOPUS.pdf http://irep.iium.edu.my/74627/19/74627_Microbial%20hydrolytic%20enzymes_wos.pdf |
| _version_ | 1848787991587192832 |
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| author | Abd Latip, Muhammad Asyraf Abdul Hamid, Azzmer Azzar Nordin, Noor Faizul Hadry |
| author_facet | Abd Latip, Muhammad Asyraf Abdul Hamid, Azzmer Azzar Nordin, Noor Faizul Hadry |
| author_sort | Abd Latip, Muhammad Asyraf |
| building | IIUM Repository |
| collection | Online Access |
| description | Enzyme is important as biocatalyst for industrial and biotechnological applications. Cold active enzymes have
showed many advantages compare to mesophilic enzymes. Their cold active and thermolabile characteristics
have shown potential benefits in many industries. In silico characterization of hydrolytic enzymes originated
from polar and tropical regions was conducted. Amino acid sequences and molecular structures of mesophilic
and psychrophilic bacterial enzyme homologues were compared thoroughly. Amino acid sequences from these
two homologs do not showed any extraordinary differences. Overall, protein folds were highly similar when
psychrophilic homologies were matched with mesophilic homologies. Active site residues located in catalytic
domain of both psychrophilic and mesophilic enzymes were highly conserved. Thus, the hydrolytic mechanisms
of these cold active enzymes still remain similar to mesophilic enzymes. However, the differences were indicated
between these two enzymes at the substrate-binding sites. Cold active enzymes showed unique loop conformations and smaller side chains at the entrances. These characteristics provided larger active site of the
enzymes. Larger entrance of active site was expected to stipulate substrates binding and products exiting with
lower energy consumption. This part of the enzymes indicated one of the important features of cold active
enzyme to work efficiently at lower temperature. Based on this in silico study, cold active enzymes have much
more advantages compared to the mesophilic enzymes that made them valuable to be further researched and
applied at industrial level. |
| first_indexed | 2025-11-14T17:33:43Z |
| format | Article |
| id | iium-74627 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:33:43Z |
| publishDate | 2019 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-746272019-11-20T09:37:09Z http://irep.iium.edu.my/74627/ Microbial hydrolytic enzymes: In silico studies between polar and tropical regions Abd Latip, Muhammad Asyraf Abdul Hamid, Azzmer Azzar Nordin, Noor Faizul Hadry QR Microbiology Enzyme is important as biocatalyst for industrial and biotechnological applications. Cold active enzymes have showed many advantages compare to mesophilic enzymes. Their cold active and thermolabile characteristics have shown potential benefits in many industries. In silico characterization of hydrolytic enzymes originated from polar and tropical regions was conducted. Amino acid sequences and molecular structures of mesophilic and psychrophilic bacterial enzyme homologues were compared thoroughly. Amino acid sequences from these two homologs do not showed any extraordinary differences. Overall, protein folds were highly similar when psychrophilic homologies were matched with mesophilic homologies. Active site residues located in catalytic domain of both psychrophilic and mesophilic enzymes were highly conserved. Thus, the hydrolytic mechanisms of these cold active enzymes still remain similar to mesophilic enzymes. However, the differences were indicated between these two enzymes at the substrate-binding sites. Cold active enzymes showed unique loop conformations and smaller side chains at the entrances. These characteristics provided larger active site of the enzymes. Larger entrance of active site was expected to stipulate substrates binding and products exiting with lower energy consumption. This part of the enzymes indicated one of the important features of cold active enzyme to work efficiently at lower temperature. Based on this in silico study, cold active enzymes have much more advantages compared to the mesophilic enzymes that made them valuable to be further researched and applied at industrial level. Elsevier 2019-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/74627/7/74627%20Microbial%20hydrolytic%20enzymes.pdf application/pdf en http://irep.iium.edu.my/74627/8/74627%20Microbial%20hydrolytic%20enzymes%20SCOPUS.pdf application/pdf en http://irep.iium.edu.my/74627/19/74627_Microbial%20hydrolytic%20enzymes_wos.pdf Abd Latip, Muhammad Asyraf and Abdul Hamid, Azzmer Azzar and Nordin, Noor Faizul Hadry (2019) Microbial hydrolytic enzymes: In silico studies between polar and tropical regions. Polar Science, 20 (part 1). pp. 9-18. ISSN 1873-9652 https://www.sciencedirect.com/science/article/abs/pii/S187396521830094X 10.1016/j.polar.2019.04.003 |
| spellingShingle | QR Microbiology Abd Latip, Muhammad Asyraf Abdul Hamid, Azzmer Azzar Nordin, Noor Faizul Hadry Microbial hydrolytic enzymes: In silico studies between polar and tropical regions |
| title | Microbial hydrolytic enzymes: In silico studies between polar and tropical regions |
| title_full | Microbial hydrolytic enzymes: In silico studies between polar and tropical regions |
| title_fullStr | Microbial hydrolytic enzymes: In silico studies between polar and tropical regions |
| title_full_unstemmed | Microbial hydrolytic enzymes: In silico studies between polar and tropical regions |
| title_short | Microbial hydrolytic enzymes: In silico studies between polar and tropical regions |
| title_sort | microbial hydrolytic enzymes: in silico studies between polar and tropical regions |
| topic | QR Microbiology |
| url | http://irep.iium.edu.my/74627/ http://irep.iium.edu.my/74627/ http://irep.iium.edu.my/74627/ http://irep.iium.edu.my/74627/7/74627%20Microbial%20hydrolytic%20enzymes.pdf http://irep.iium.edu.my/74627/8/74627%20Microbial%20hydrolytic%20enzymes%20SCOPUS.pdf http://irep.iium.edu.my/74627/19/74627_Microbial%20hydrolytic%20enzymes_wos.pdf |