Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains
A lab scale study was carried out to produce cellulase enzyme using two types of media: Media B consist of domestic wastewater sludge (DWS), microgranullar cellulose and oil palm empty fruit bunch (EFB) and Media M consist of DWS, microgranullar cellulose, oil palm EFB and addition of methanol. The...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
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2010
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| Online Access: | http://irep.iium.edu.my/5583/ http://irep.iium.edu.my/5583/1/Full-paper-Bangkok.pdf |
| _version_ | 1848776530299191296 |
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| author | Alam, Md. Zahangir Drani, Afifah Muyibi, Suleyman Aremu |
| author_facet | Alam, Md. Zahangir Drani, Afifah Muyibi, Suleyman Aremu |
| author_sort | Alam, Md. Zahangir |
| building | IIUM Repository |
| collection | Online Access |
| description | A lab scale study was carried out to produce cellulase enzyme using two types of media: Media B consist of domestic wastewater sludge (DWS), microgranullar cellulose and oil palm empty fruit bunch (EFB) and Media M consist of DWS, microgranullar cellulose, oil palm EFB and addition of methanol. The 3% (v/v) inoculum of Trichoderma reesei Rut C-30 (ATCC 56765) and locally isolated Aspergillus spp. O-102A was inoculated to both media. The maximum total cellulase activity produced was 0.284 FPU/ml (Day 5) and endo-β-1,4 glucanase (CMCase) activity 2.631 IU/ml (Day 7) by T.reesei in media B. The cellulase of T.reesei was higher than O-102A, which only 0.093 FPU/ml (total cellulase activity) and 0.576 IU/ml (CMCase activity) produced by O-102A on the same day of fermentation in media B. The addition of methanol to the substrate (media M) was showing no positive effect to the production of cellulase enzyme for both strains. |
| first_indexed | 2025-11-14T14:31:33Z |
| format | Proceeding Paper |
| id | iium-5583 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:31:33Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-55832012-01-17T04:05:58Z http://irep.iium.edu.my/5583/ Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains Alam, Md. Zahangir Drani, Afifah Muyibi, Suleyman Aremu TP Chemical technology TP248.13 Biotechnology A lab scale study was carried out to produce cellulase enzyme using two types of media: Media B consist of domestic wastewater sludge (DWS), microgranullar cellulose and oil palm empty fruit bunch (EFB) and Media M consist of DWS, microgranullar cellulose, oil palm EFB and addition of methanol. The 3% (v/v) inoculum of Trichoderma reesei Rut C-30 (ATCC 56765) and locally isolated Aspergillus spp. O-102A was inoculated to both media. The maximum total cellulase activity produced was 0.284 FPU/ml (Day 5) and endo-β-1,4 glucanase (CMCase) activity 2.631 IU/ml (Day 7) by T.reesei in media B. The cellulase of T.reesei was higher than O-102A, which only 0.093 FPU/ml (total cellulase activity) and 0.576 IU/ml (CMCase activity) produced by O-102A on the same day of fermentation in media B. The addition of methanol to the substrate (media M) was showing no positive effect to the production of cellulase enzyme for both strains. 2010-04-23 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/5583/1/Full-paper-Bangkok.pdf Alam, Md. Zahangir and Drani, Afifah and Muyibi, Suleyman Aremu (2010) Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains. In: International Conference on Environmental Science and Technology (ICEST 2010) , 23-25 April 2010, Bangkok, Thailand. (Unpublished) |
| spellingShingle | TP Chemical technology TP248.13 Biotechnology Alam, Md. Zahangir Drani, Afifah Muyibi, Suleyman Aremu Domestic wastewater sludge as a major substrate for cellulase production by two different fungal strains |
| title | Domestic wastewater sludge as a major substrate for cellulase
production by two different fungal strains |
| title_full | Domestic wastewater sludge as a major substrate for cellulase
production by two different fungal strains |
| title_fullStr | Domestic wastewater sludge as a major substrate for cellulase
production by two different fungal strains |
| title_full_unstemmed | Domestic wastewater sludge as a major substrate for cellulase
production by two different fungal strains |
| title_short | Domestic wastewater sludge as a major substrate for cellulase
production by two different fungal strains |
| title_sort | domestic wastewater sludge as a major substrate for cellulase
production by two different fungal strains |
| topic | TP Chemical technology TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/5583/ http://irep.iium.edu.my/5583/1/Full-paper-Bangkok.pdf |