Monoclonal antibody production: media optimization for
enhancement the cell viability of hybridoma cell

QR Code

Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell

Media optimization of RCI hybridoma cell culture for monoclonal antibody production was carried out in T -Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology(STATISTICA v 6.1) has show...

Full description

Bibliographic Details
Main Authors:	Mel, Maizirwan, Mat Saad, Maizura, Hashim, Yumi Zuhanis Has-Yun, Mohamed Salleh, Moharnad Ramlan
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2008
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/5476/ http://irep.iium.edu.my/5476/1/1957-AJSR_%28Published_On-Line%29.pdf

Similar Items

Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor
by: Mel, Maizirwan, et al.
Published: (2008)

Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture
by: Arifin, Mohd Azmir, et al.
Published: (2015)

Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)

Pathway analysis of genes affected in MCF-7 breast cancer cells treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)

CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost
by: Packeer Mohamed, Vasila, et al.
Published: (2011)

Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system
by: Packeer Mohamed, Vasila, et al.
Published: (2011)

Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor
by: Mel, Maizirwan, et al.
Published: (2005)

Effects of different types of growth media on metabolite profiles (GCMS-based) of CHO-K1 cells expressing IGF-1 proteins
by: Mohamed Saberi, Salfarina Ezrina, et al.
Published: (2011)

Different oxidation treatments on polystyrene (PS) microspheres by using an ultraviolet/ozone (UVO3) system
by: Mel, Maizirwan, et al.
Published: (2015)

Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor
by: Mel, Maizirwan, et al.
Published: (2011)

Microcarriers for cell culture technology
by: Mel, Maizirwan, et al.
Published: (2011)

Effect of commercial bromelain on viability of MCF-7 human breast cancer cells
by: Yahaya, Hanan, et al.
Published: (2013)

Development of new media formulation for the improvement of recombinant protein expression in E.Coli
by: Mohd. Salleh, Hamzah, et al.
Published: (2012)

In vitro cancer cell line classification using pattern recognition approach based on metabolite profiling
by: Jeffree, Amanina Iymia, et al.
Published: (2018)

Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor
by: Mel, Maizirwan, et al.
Published: (2011)

Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
by: Mel, Maizirwan, et al.
Published: (2010)

Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds
by: Abd Rahim, Toibah, et al.
Published: (2016)

Efficient performance of locally processed serum in vero cell culture
by: Ahmad Nor, Yusilawati, et al.
Published: (2011)

In vitro assay for investigating potential anti-cancer agents targeting at metastatic level
by: Hashim, Yumi Zuhanis Has-Yun, et al.
Published: (2011)

Screening of anticancer activity from agarwood essential oil
by: Hashim, Yumi Zuhanis Has-Yun, et al.
Published: (2014)

Modification of Polycaprolactone microspheres surface by ultra violet/ozone treatment to improve its hydrophilicity
by: Samsudin, Nurhusna, et al.
Published: (2015)

Cell rupture of recombinant escherichia coli using high pressure homogenizer
by: Ahmad Raus, Raha, et al.
Published: (2010)

The growth study of vero cells in different type of microcarrier
by: Ahmad Nor, Yusilawati, et al.
Published: (2010)

Autologous human serum supports better proliferation, viability and homogeneity of peripheral blood mononuclear cells over fetal bovine serum
by: Haque, Nazmul, et al.
Published: (2014)

Anti-inflammatory activity exhibited by supercritical fluid extract of aquilaria malaccensis leaves
by: Eissa, Manar, et al.
Published: (2018)

Agarwood (A. malaccensis) leaf as an alternative Halal source of anti-inflammatory compounds
by: Zainurin, Nur Aimi Aliah, et al.
Published: (2018)

Serum free medium
by: Arifin, Mohd Azmir, et al.
Published: (2011)

Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture
by: Ahmad Nor, Yusilawati, et al.
Published: (2010)

Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells
by: Abbas, Phirdaous, et al.
Published: (2019)

Fabrication and characterization of plant mediated green zinc nanoparticles for antileishmanial properties
by: Gul, Safia, et al.
Published: (2019)

Cell disruption improvement of E. Coli producing NP-AIV using high pressure homogenizer
by: Mel, Maizirwan, et al.
Published: (2011)

Comparison of alcohol free GSH production by ultrasonic and homogenizing method
by: Mohamed Salleh, Mohamad Ramlan, et al.
Published: (2008)

Optimizing media of lactobacillus rhamnosus for lactic acid fermentation
by: Mel, Maizirwan, et al.
Published: (2008)

Development of porous PCL-based microcarrier
by: Mohd. Salleh, Hamzah, et al.
Published: (2018)

Metabolite profiling of leaves and branch of Aquilaria sp.
by: Mohamed Azmin, Nor Fadhillah, et al.
Published: (2018)

Bioprocessing of recombinant E.Coli producing Glucuronidase Enzymes
by: Mel, Maizirwan
Published: (2012)

Agarwood leaf essential oil characterization and effects on MCF-7 breast cancer cells
by: Zainurin, Nur Aimi Aliah, et al.
Published: (2018)

Investigation of the viability of selected microorganisms on the biodegradation of Palm Oil Mill Effluent(POME)
by: Abass Olanrewaju, Alade, et al.
Published: (2012)

Surface modification of microporous polycaprolactone (PCL) microcarrier to improve microcarrier biocompatibility
by: Samsudin, Nurhusna, et al.
Published: (2018)

Modes of bioreactor operations
by: Mel, Maizirwan, et al.
Published: (2011)