Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
Halal slaughtered chicken is a major concern among Muslims. Currently,stunning methods has been adopted worldwide aiming at maximizing production. However, stunning treatment which is not in compliance with the Islamic teaching will end up with unlawful chickens for Muslim consumption. In this study...
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| Format: | Book Chapter |
| Language: | English |
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Springer Singapore
2016
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| Online Access: | http://irep.iium.edu.my/51398/ http://irep.iium.edu.my/51398/1/51398_Identification%20of%20aldolase%20C%2C%20actin-related%20protein%202%20homolog%2C%20and%20enolase%201%20as%20potential%20biomarkers%20for%20electrically%20stunned%20chickens.pdf |
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| author | Abu Samah, Norshahida Amid, Azura Yusof, Faridah |
| author2 | Ab. Manan, Siti Khadijah |
| author_facet | Ab. Manan, Siti Khadijah Abu Samah, Norshahida Amid, Azura Yusof, Faridah |
| author_sort | Abu Samah, Norshahida |
| building | IIUM Repository |
| collection | Online Access |
| description | Halal slaughtered chicken is a major concern among Muslims. Currently,stunning methods has been adopted worldwide aiming at maximizing production. However, stunning treatment which is not in compliance with the Islamic teaching will end up with unlawful chickens for Muslim consumption. In this study, proteomics methods have been used to identify potential biomarkers for the effects of electrical stimulation on the protein profiles of chicken’s brain. The protein profiles were constructed in order to detect any differences in the protein expression and modifications. The electrical stimulation was conducted with different current and voltage. The different voltages studied were 10, 40, and 70 V while the values for current studied were 0.25, 0.5, and 0.75 A. The profiles from these electrically stimulated chicken were compared to the 0 A, 0 V non-stunned sample. After the proteomics analyses using 2D Platinum ImageMaster 6.0 and matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF) identification, aldolase C, actin-related protein 2 homolog (ARP2), and enolase 1 were identified to be positively present in the brain sample of electrically stimulated samples. The over expression of the proteins were further confirmed at the transcriptional level by real-time PCR. Results from MALDI-TOF and real-time PCRwere in agreement. Therefore, this identification of biomarkers for electrically
stimulated chicken’s brain provides a novel approach on differentiating the stunned chickens from the non-stunned ones. |
| first_indexed | 2025-11-14T16:27:50Z |
| format | Book Chapter |
| id | iium-51398 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:27:50Z |
| publishDate | 2016 |
| publisher | Springer Singapore |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-513982016-10-24T04:28:02Z http://irep.iium.edu.my/51398/ Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens Abu Samah, Norshahida Amid, Azura Yusof, Faridah TP248.13 Biotechnology Halal slaughtered chicken is a major concern among Muslims. Currently,stunning methods has been adopted worldwide aiming at maximizing production. However, stunning treatment which is not in compliance with the Islamic teaching will end up with unlawful chickens for Muslim consumption. In this study, proteomics methods have been used to identify potential biomarkers for the effects of electrical stimulation on the protein profiles of chicken’s brain. The protein profiles were constructed in order to detect any differences in the protein expression and modifications. The electrical stimulation was conducted with different current and voltage. The different voltages studied were 10, 40, and 70 V while the values for current studied were 0.25, 0.5, and 0.75 A. The profiles from these electrically stimulated chicken were compared to the 0 A, 0 V non-stunned sample. After the proteomics analyses using 2D Platinum ImageMaster 6.0 and matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF) identification, aldolase C, actin-related protein 2 homolog (ARP2), and enolase 1 were identified to be positively present in the brain sample of electrically stimulated samples. The over expression of the proteins were further confirmed at the transcriptional level by real-time PCR. Results from MALDI-TOF and real-time PCRwere in agreement. Therefore, this identification of biomarkers for electrically stimulated chicken’s brain provides a novel approach on differentiating the stunned chickens from the non-stunned ones. Springer Singapore Ab. Manan, Siti Khadijah Abd Rahman, Fadilah Sahri, Mardhiyyah 2016 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/51398/1/51398_Identification%20of%20aldolase%20C%2C%20actin-related%20protein%202%20homolog%2C%20and%20enolase%201%20as%20potential%20biomarkers%20for%20electrically%20stunned%20chickens.pdf Abu Samah, Norshahida and Amid, Azura and Yusof, Faridah (2016) Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens. In: Contemporary issues and development in the global Halal industry: selected papers from the International Halal Conference 2014. Springer Singapore, Singapore, pp. 519-530. ISBN 978-981-10-1450-5 http://link.springer.com/chapter/10.1007%2F978-981-10-1452-9_47 |
| spellingShingle | TP248.13 Biotechnology Abu Samah, Norshahida Amid, Azura Yusof, Faridah Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens |
| title | Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens |
| title_full | Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens |
| title_fullStr | Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens |
| title_full_unstemmed | Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens |
| title_short | Identification of aldolase C, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens |
| title_sort | identification of aldolase c, actin-related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/51398/ http://irep.iium.edu.my/51398/ http://irep.iium.edu.my/51398/1/51398_Identification%20of%20aldolase%20C%2C%20actin-related%20protein%202%20homolog%2C%20and%20enolase%201%20as%20potential%20biomarkers%20for%20electrically%20stunned%20chickens.pdf |