Identification and phylogenetic inference in different mollucs nudibranch species via mitochondrial 16S rDNA

The molecular analysis of marine life is an essential approach to discover new classes of natural products and to improve the management and sustainable utilization of these useful genetic resources. Mitochondrial DNA markers are frequently utilized to identify any organism up to the species level....

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Main Authors: Alqudah, Ali, Saad, Shahbudin, Nordin, Noor Faizul Hadry, Darnis, Deny Susanti
Format: Article
Language:English
Published: Rede Brasileira de Informações Biológicas - Rebibio 2015
Subjects:
Online Access:http://irep.iium.edu.my/48405/
http://irep.iium.edu.my/48405/1/Brazilian_Journal_Biol_Sci._4.295-302.pdf
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author Alqudah, Ali
Saad, Shahbudin
Nordin, Noor Faizul Hadry
Darnis, Deny Susanti
author_facet Alqudah, Ali
Saad, Shahbudin
Nordin, Noor Faizul Hadry
Darnis, Deny Susanti
author_sort Alqudah, Ali
building IIUM Repository
collection Online Access
description The molecular analysis of marine life is an essential approach to discover new classes of natural products and to improve the management and sustainable utilization of these useful genetic resources. Mitochondrial DNA markers are frequently utilized to identify any organism up to the species level. This study provides insight into the significant role of species discrimination based on 16S rDNA, and this method could be a powerful tool for the identification of animal species. 16S rDNA sequences of each studied species (Phyllidia varicosa (Lamarck, 1801) and Phyllidiella pustulosa (Cuvier, 1804)) from two locations in the coastal waters of Balok in Pahang State and Bidong Island in Terengganu State were aligned to identify the phylogenetic relationships among them. The phylogenetic tree produced in this study is consistent with those previously presented in the literature. The divergence of sequences was adequate to identify the samples at the species level with the assistance of the GenBank database and the BLAST tool. The results presented here provide useful information for a better understanding of this marine genetic resource. This genetic approach could simplify the identification of the species accurately for further analysis.
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spelling iium-484052016-07-17T08:46:44Z http://irep.iium.edu.my/48405/ Identification and phylogenetic inference in different mollucs nudibranch species via mitochondrial 16S rDNA Alqudah, Ali Saad, Shahbudin Nordin, Noor Faizul Hadry Darnis, Deny Susanti Q Science (General) The molecular analysis of marine life is an essential approach to discover new classes of natural products and to improve the management and sustainable utilization of these useful genetic resources. Mitochondrial DNA markers are frequently utilized to identify any organism up to the species level. This study provides insight into the significant role of species discrimination based on 16S rDNA, and this method could be a powerful tool for the identification of animal species. 16S rDNA sequences of each studied species (Phyllidia varicosa (Lamarck, 1801) and Phyllidiella pustulosa (Cuvier, 1804)) from two locations in the coastal waters of Balok in Pahang State and Bidong Island in Terengganu State were aligned to identify the phylogenetic relationships among them. The phylogenetic tree produced in this study is consistent with those previously presented in the literature. The divergence of sequences was adequate to identify the samples at the species level with the assistance of the GenBank database and the BLAST tool. The results presented here provide useful information for a better understanding of this marine genetic resource. This genetic approach could simplify the identification of the species accurately for further analysis. Rede Brasileira de Informações Biológicas - Rebibio 2015-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/48405/1/Brazilian_Journal_Biol_Sci._4.295-302.pdf Alqudah, Ali and Saad, Shahbudin and Nordin, Noor Faizul Hadry and Darnis, Deny Susanti (2015) Identification and phylogenetic inference in different mollucs nudibranch species via mitochondrial 16S rDNA. Brazilian Journal of Biological Sciences, 2 (4). pp. 295-302. ISSN 2358-2731 http://revista.rebibio.net/v2n4/v02n04a12a.html
spellingShingle Q Science (General)
Alqudah, Ali
Saad, Shahbudin
Nordin, Noor Faizul Hadry
Darnis, Deny Susanti
Identification and phylogenetic inference in different mollucs nudibranch species via mitochondrial 16S rDNA
title Identification and phylogenetic inference in different mollucs nudibranch species via mitochondrial 16S rDNA
title_full Identification and phylogenetic inference in different mollucs nudibranch species via mitochondrial 16S rDNA
title_fullStr Identification and phylogenetic inference in different mollucs nudibranch species via mitochondrial 16S rDNA
title_full_unstemmed Identification and phylogenetic inference in different mollucs nudibranch species via mitochondrial 16S rDNA
title_short Identification and phylogenetic inference in different mollucs nudibranch species via mitochondrial 16S rDNA
title_sort identification and phylogenetic inference in different mollucs nudibranch species via mitochondrial 16s rdna
topic Q Science (General)
url http://irep.iium.edu.my/48405/
http://irep.iium.edu.my/48405/
http://irep.iium.edu.my/48405/1/Brazilian_Journal_Biol_Sci._4.295-302.pdf