Constitutive, institutive and up-regulation of carotenogenesis regulatory mechanism via in vitro culture model system and elicitors

Phytohormone abscisic acid (ABA) plays a regulatory role in many physiological processes in plants and is regulated and controlled by specific key factors or genes. Different environmental stress conditions such as water, drought, cold, light, and temperature result in increased amounts of ABA. Th...

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Main Authors: Othman, Rashidi, Mohd Hassan, Norazian, Mohd Zaifuddin, Fatimah Azzahra
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia (UKM) 2015
Subjects:
Online Access:http://irep.iium.edu.my/43475/
http://irep.iium.edu.my/43475/1/MJAS_2.pdf
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author Othman, Rashidi
Mohd Hassan, Norazian
Mohd Zaifuddin, Fatimah Azzahra
author_facet Othman, Rashidi
Mohd Hassan, Norazian
Mohd Zaifuddin, Fatimah Azzahra
author_sort Othman, Rashidi
building IIUM Repository
collection Online Access
description Phytohormone abscisic acid (ABA) plays a regulatory role in many physiological processes in plants and is regulated and controlled by specific key factors or genes. Different environmental stress conditions such as water, drought, cold, light, and temperature result in increased amounts of ABA. The action of ABA involves modification of gene expression and analysis of in vitro callus model system cultures revealed several potential of constitutive, institutive and up-regulation acting regulatory mechanisms. Therefore, this study was aimed at establishing in vitro cultures as potential research tools to study the regulatory mechanisms of the carotenoid biosynthesis in selected plant species through a controlled environment. The presence and absence of zeaxanthin and neoxanthin in callus cultures and intact plants could be explained by changes in gene expression in response to stress. Abiotic stress can alter gene expression and trigger cellular metabolism in plants. This study suggested that the key factors which involved in regulatory mechanisms of individual carotenoid biosynthesis in a particular biology system of plants can be either be silenced or activated. Therefore, based on the results in this study environmental stress is made possible for enhancement or enrichment of certain carotenoid of interest in food crops without altering the genes.
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spelling iium-434752021-07-23T15:59:02Z http://irep.iium.edu.my/43475/ Constitutive, institutive and up-regulation of carotenogenesis regulatory mechanism via in vitro culture model system and elicitors Othman, Rashidi Mohd Hassan, Norazian Mohd Zaifuddin, Fatimah Azzahra SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Phytohormone abscisic acid (ABA) plays a regulatory role in many physiological processes in plants and is regulated and controlled by specific key factors or genes. Different environmental stress conditions such as water, drought, cold, light, and temperature result in increased amounts of ABA. The action of ABA involves modification of gene expression and analysis of in vitro callus model system cultures revealed several potential of constitutive, institutive and up-regulation acting regulatory mechanisms. Therefore, this study was aimed at establishing in vitro cultures as potential research tools to study the regulatory mechanisms of the carotenoid biosynthesis in selected plant species through a controlled environment. The presence and absence of zeaxanthin and neoxanthin in callus cultures and intact plants could be explained by changes in gene expression in response to stress. Abiotic stress can alter gene expression and trigger cellular metabolism in plants. This study suggested that the key factors which involved in regulatory mechanisms of individual carotenoid biosynthesis in a particular biology system of plants can be either be silenced or activated. Therefore, based on the results in this study environmental stress is made possible for enhancement or enrichment of certain carotenoid of interest in food crops without altering the genes. Universiti Kebangsaan Malaysia (UKM) 2015-01-14 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43475/1/MJAS_2.pdf Othman, Rashidi and Mohd Hassan, Norazian and Mohd Zaifuddin, Fatimah Azzahra (2015) Constitutive, institutive and up-regulation of carotenogenesis regulatory mechanism via in vitro culture model system and elicitors. Malaysian Journal of Analytical Sciences (MJAS), 19 (2). pp. 437-444. ISSN 1394-2506 http://www.ukm.my/mjas/
spellingShingle SB Plant culture
TP248.13 Biotechnology
TP934 Paints, pigments, varnishes, etc
Othman, Rashidi
Mohd Hassan, Norazian
Mohd Zaifuddin, Fatimah Azzahra
Constitutive, institutive and up-regulation of carotenogenesis regulatory mechanism via in vitro culture model system and elicitors
title Constitutive, institutive and up-regulation of carotenogenesis regulatory mechanism via in vitro culture model system and elicitors
title_full Constitutive, institutive and up-regulation of carotenogenesis regulatory mechanism via in vitro culture model system and elicitors
title_fullStr Constitutive, institutive and up-regulation of carotenogenesis regulatory mechanism via in vitro culture model system and elicitors
title_full_unstemmed Constitutive, institutive and up-regulation of carotenogenesis regulatory mechanism via in vitro culture model system and elicitors
title_short Constitutive, institutive and up-regulation of carotenogenesis regulatory mechanism via in vitro culture model system and elicitors
title_sort constitutive, institutive and up-regulation of carotenogenesis regulatory mechanism via in vitro culture model system and elicitors
topic SB Plant culture
TP248.13 Biotechnology
TP934 Paints, pigments, varnishes, etc
url http://irep.iium.edu.my/43475/
http://irep.iium.edu.my/43475/
http://irep.iium.edu.my/43475/1/MJAS_2.pdf