Thermostable lipases: an overview of production, purification and characterization
Thermostable lipases occupy a prominent position in aqueous and non-aqueous biocatalysis. Isolation of wild strains with novel properties has been on-going and recombinant strains are being constructed so as to meet the biotechnological applications of lipases. Different purification methods prove...
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| Format: | Article |
| Language: | English English |
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Oriental Scientific Publishing Company
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/40933/ http://irep.iium.edu.my/40933/1/Paper-aliyu.pdf http://irep.iium.edu.my/40933/4/40933-Thermostable%20lipases_SCOPUS.pdf |
| _version_ | 1848782029180633088 |
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| author | Salihu, Aliyu Alam, Md Zahangir |
| author_facet | Salihu, Aliyu Alam, Md Zahangir |
| author_sort | Salihu, Aliyu |
| building | IIUM Repository |
| collection | Online Access |
| description | Thermostable lipases occupy a prominent position in aqueous and non-aqueous biocatalysis. Isolation of wild strains with novel properties has been on-going and
recombinant strains are being constructed so as to meet the biotechnological applications of lipases. Different purification methods prove effective and the purity of the enzyme is dependent on the intended applications. Unlike medical and pharmaceutical applications
where highly pure preparations are needed, partial purification can be sufficient for general applications. Thus, characterization associated with enzyme activity, specificity, thermostability, enatioselectivity and tolerance to various solvent systems are the prerequisite for thermostable lipase selection which make them highly demanding in lipase catalyzed reactions. This article intends to give an insight on thermostable lipases so as to stimulate researchers to explore other unique properties from wild and recombinant strains that could be of benefit for wider industrial applications. |
| first_indexed | 2025-11-14T15:58:57Z |
| format | Article |
| id | iium-40933 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T15:58:57Z |
| publishDate | 2014 |
| publisher | Oriental Scientific Publishing Company |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-409332017-08-10T07:49:02Z http://irep.iium.edu.my/40933/ Thermostable lipases: an overview of production, purification and characterization Salihu, Aliyu Alam, Md Zahangir TP248.13 Biotechnology Thermostable lipases occupy a prominent position in aqueous and non-aqueous biocatalysis. Isolation of wild strains with novel properties has been on-going and recombinant strains are being constructed so as to meet the biotechnological applications of lipases. Different purification methods prove effective and the purity of the enzyme is dependent on the intended applications. Unlike medical and pharmaceutical applications where highly pure preparations are needed, partial purification can be sufficient for general applications. Thus, characterization associated with enzyme activity, specificity, thermostability, enatioselectivity and tolerance to various solvent systems are the prerequisite for thermostable lipase selection which make them highly demanding in lipase catalyzed reactions. This article intends to give an insight on thermostable lipases so as to stimulate researchers to explore other unique properties from wild and recombinant strains that could be of benefit for wider industrial applications. Oriental Scientific Publishing Company 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/40933/1/Paper-aliyu.pdf application/pdf en http://irep.iium.edu.my/40933/4/40933-Thermostable%20lipases_SCOPUS.pdf Salihu, Aliyu and Alam, Md Zahangir (2014) Thermostable lipases: an overview of production, purification and characterization. Biosciences Biotechnology Research Asia, 11 (3). pp. 1095-1107. ISSN 0973-1245 http://www.biotech-asia.org/index.php 10.13005/bbra/1494 |
| spellingShingle | TP248.13 Biotechnology Salihu, Aliyu Alam, Md Zahangir Thermostable lipases: an overview of production, purification and characterization |
| title | Thermostable lipases: an overview of production, purification and characterization |
| title_full | Thermostable lipases: an overview of production, purification and characterization |
| title_fullStr | Thermostable lipases: an overview of production, purification and characterization |
| title_full_unstemmed | Thermostable lipases: an overview of production, purification and characterization |
| title_short | Thermostable lipases: an overview of production, purification and characterization |
| title_sort | thermostable lipases: an overview of production, purification and characterization |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/40933/ http://irep.iium.edu.my/40933/ http://irep.iium.edu.my/40933/ http://irep.iium.edu.my/40933/1/Paper-aliyu.pdf http://irep.iium.edu.my/40933/4/40933-Thermostable%20lipases_SCOPUS.pdf |