Process optimization of glucoamylase production by aspergillus niger using low cost bitter cassava (manihot esculenta)

The effect of process parameters on glucoamylase production from bitter cassava by Aspergillus niger was studied using response surface methodology (RSM). Face centered central composite design (FCCCD) was selected to evaluate the optimum levels of the three param...

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Main Authors: Alam, Md Zahangir, Khalaf, Abubakir I., Mohd. Salleh, Hamzah, Salihu, Aliyu
Format: Article
Language:English
Published: AENSI Publications 2014
Subjects:
Online Access:http://irep.iium.edu.my/39801/
http://irep.iium.edu.my/39801/1/Paper-amylase-abu_bakir.pdf
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author Alam, Md Zahangir
Khalaf, Abubakir I.
Mohd. Salleh, Hamzah
Salihu, Aliyu
author_facet Alam, Md Zahangir
Khalaf, Abubakir I.
Mohd. Salleh, Hamzah
Salihu, Aliyu
author_sort Alam, Md Zahangir
building IIUM Repository
collection Online Access
description The effect of process parameters on glucoamylase production from bitter cassava by Aspergillus niger was studied using response surface methodology (RSM). Face centered central composite design (FCCCD) was selected to evaluate the optimum levels of the three parameters (pH, inoculum concentration and agitation speed) and the developed quadratic model was found to be significant with p-value < 0.0001. The most important parameter was pH based on its lowest p-value of < 0.0001 and the optimal combinations of the selected parameters for maximum glucoamylase production were found to be 4.8, 3.7% (v/v) and 260 rpm for pH, inoculum concentration and agitation speed respectively. The model R2 value of 0.9905 showed the fitness of the experimental response with the predicted ones. Thus, this study develops an efficient fermentation system using bitter cassava for enhanced production of glucoamylase production by A. nige
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institution International Islamic University Malaysia
institution_category Local University
language English
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publishDate 2014
publisher AENSI Publications
recordtype eprints
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spelling iium-398012021-07-23T01:41:12Z http://irep.iium.edu.my/39801/ Process optimization of glucoamylase production by aspergillus niger using low cost bitter cassava (manihot esculenta) Alam, Md Zahangir Khalaf, Abubakir I. Mohd. Salleh, Hamzah Salihu, Aliyu TP248.13 Biotechnology The effect of process parameters on glucoamylase production from bitter cassava by Aspergillus niger was studied using response surface methodology (RSM). Face centered central composite design (FCCCD) was selected to evaluate the optimum levels of the three parameters (pH, inoculum concentration and agitation speed) and the developed quadratic model was found to be significant with p-value < 0.0001. The most important parameter was pH based on its lowest p-value of < 0.0001 and the optimal combinations of the selected parameters for maximum glucoamylase production were found to be 4.8, 3.7% (v/v) and 260 rpm for pH, inoculum concentration and agitation speed respectively. The model R2 value of 0.9905 showed the fitness of the experimental response with the predicted ones. Thus, this study develops an efficient fermentation system using bitter cassava for enhanced production of glucoamylase production by A. nige AENSI Publications 2014-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39801/1/Paper-amylase-abu_bakir.pdf Alam, Md Zahangir and Khalaf, Abubakir I. and Mohd. Salleh, Hamzah and Salihu, Aliyu (2014) Process optimization of glucoamylase production by aspergillus niger using low cost bitter cassava (manihot esculenta). Advances in Environmental Biology, 8 (17). pp. 42-47. ISSN 1995-0756 http://www.aensiweb.com/old/aeb/September%202014/42-47.pdf
spellingShingle TP248.13 Biotechnology
Alam, Md Zahangir
Khalaf, Abubakir I.
Mohd. Salleh, Hamzah
Salihu, Aliyu
Process optimization of glucoamylase production by aspergillus niger using low cost bitter cassava (manihot esculenta)
title Process optimization of glucoamylase production by aspergillus niger using low cost bitter cassava (manihot esculenta)
title_full Process optimization of glucoamylase production by aspergillus niger using low cost bitter cassava (manihot esculenta)
title_fullStr Process optimization of glucoamylase production by aspergillus niger using low cost bitter cassava (manihot esculenta)
title_full_unstemmed Process optimization of glucoamylase production by aspergillus niger using low cost bitter cassava (manihot esculenta)
title_short Process optimization of glucoamylase production by aspergillus niger using low cost bitter cassava (manihot esculenta)
title_sort process optimization of glucoamylase production by aspergillus niger using low cost bitter cassava (manihot esculenta)
topic TP248.13 Biotechnology
url http://irep.iium.edu.my/39801/
http://irep.iium.edu.my/39801/
http://irep.iium.edu.my/39801/1/Paper-amylase-abu_bakir.pdf