Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger
A number of media components influencing a-amylase production by Aspergillus niger O103A were studied using local bitter variety of cassava (Manihotes cuelenta) as the main substrate. The media components (cassava, maltose, glucose, yeast extract, urea, NH4NO3, KH2PO4, CaCl2, MgSO4.7H2O, NaCl, and F...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2014
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| Online Access: | http://irep.iium.edu.my/38273/ http://irep.iium.edu.my/38273/1/Paper-JPAM-abubakir.pdf |
| _version_ | 1848781575042367488 |
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| author | Khalaf, Abubakir I. Alam, Md Zahangir Mohd. Salleh, Hamzah |
| author_facet | Khalaf, Abubakir I. Alam, Md Zahangir Mohd. Salleh, Hamzah |
| author_sort | Khalaf, Abubakir I. |
| building | IIUM Repository |
| collection | Online Access |
| description | A number of media components influencing a-amylase production by Aspergillus niger O103A were studied using local bitter variety of cassava (Manihotes cuelenta) as the main substrate. The media components (cassava, maltose, glucose, yeast extract, urea, NH4NO3, KH2PO4, CaCl2, MgSO4.7H2O, NaCl, and FeSO4.7H2O) were analyzed using Plackett?Burman design. Optimal concentrations of the components contributing positively to enzyme production were further determined using one factor at a time (OFAT) and response surface methodology (RSM) method. Among different nitrogen and supplementary carbon sources tested, yeast extract 2 g/l and maltose 3 g/L were most effective for maximum production of enzyme (49 U/ml). The results show a favorable a-amylase production by applying statistical approach in optimization process. |
| first_indexed | 2025-11-14T15:51:44Z |
| format | Article |
| id | iium-38273 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:51:44Z |
| publishDate | 2014 |
| publisher | Journal of Pure and Applied Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-382732021-07-23T01:48:48Z http://irep.iium.edu.my/38273/ Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger Khalaf, Abubakir I. Alam, Md Zahangir Mohd. Salleh, Hamzah TP248.13 Biotechnology A number of media components influencing a-amylase production by Aspergillus niger O103A were studied using local bitter variety of cassava (Manihotes cuelenta) as the main substrate. The media components (cassava, maltose, glucose, yeast extract, urea, NH4NO3, KH2PO4, CaCl2, MgSO4.7H2O, NaCl, and FeSO4.7H2O) were analyzed using Plackett?Burman design. Optimal concentrations of the components contributing positively to enzyme production were further determined using one factor at a time (OFAT) and response surface methodology (RSM) method. Among different nitrogen and supplementary carbon sources tested, yeast extract 2 g/l and maltose 3 g/L were most effective for maximum production of enzyme (49 U/ml). The results show a favorable a-amylase production by applying statistical approach in optimization process. Journal of Pure and Applied Microbiology 2014-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38273/1/Paper-JPAM-abubakir.pdf Khalaf, Abubakir I. and Alam, Md Zahangir and Mohd. Salleh, Hamzah (2014) Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger. Journal of Pure and Applied Microbiology, 8 (s.ed 1). pp. 719-724. ISSN 0973-7510 http://www.microbiologyjournal.org/ |
| spellingShingle | TP248.13 Biotechnology Khalaf, Abubakir I. Alam, Md Zahangir Mohd. Salleh, Hamzah Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title | Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title_full | Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title_fullStr | Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title_full_unstemmed | Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title_short | Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title_sort | optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/38273/ http://irep.iium.edu.my/38273/ http://irep.iium.edu.my/38273/1/Paper-JPAM-abubakir.pdf |