In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd
Medicinal use of Acrostichum aureum and A. speciosum in wound healing treatment by Malay community was tested scientifically in this project. Wound healing properties of ethanolic, aqueous extracts and the fractions of A. aureum and A. speciosum were evaluated in vitro using scratch wound assay on N...
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| Format: | Proceeding Paper |
| Language: | English |
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Ibnu Sina Institute, UTM
2012
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| Online Access: | http://irep.iium.edu.my/27426/ http://irep.iium.edu.my/27426/1/ISPC_2012_PROCEEDING_BOOK.pdf |
| _version_ | 1848779817531473920 |
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| author | Herman, Hendy Putra Darnis, Deny Susanti Saad, Shahbudin Bakhtiar, M. Taher Ramli, Norazsida |
| author_facet | Herman, Hendy Putra Darnis, Deny Susanti Saad, Shahbudin Bakhtiar, M. Taher Ramli, Norazsida |
| author_sort | Herman, Hendy Putra |
| building | IIUM Repository |
| collection | Online Access |
| description | Medicinal use of Acrostichum aureum and A. speciosum in wound healing treatment by Malay community was tested scientifically in this project. Wound healing properties of ethanolic, aqueous extracts and the fractions of A. aureum and A. speciosum were evaluated in vitro using scratch wound assay on NIH/3T3 cell line. In total, 22 different extracts and fractions from leaves, stems, and rhizomes of both species were used for this testing. NIH/
3T3 cell lines were seeded at a density of 5 x 104 cells/ mL in 24-well plate and incubated at 37°C until reaching nearly confluence. The confluent cell monolayer was manually scratched with a sterile pipette tip. This test was done in triplicate. For the treatment, each well was treated with 0.2 mg/ mL of the different extracts, 2 ng/ mL of platelet derived growth factor (PDGF) as
positive control and DMEM medium as negative control. The images before treatment and after 12 hour post-treatment were photographed using ProgRes® CapturePro 2.5 software to measure mean migration rate (μm/hour) and open image area (%) using TScratch software. The results
showed that cell line treated with aqueous extract of rhizomes and stems of both species have improved the migration rate. The fractions of A. aureum (n-Hexane, n- Hexane: DCM, and ethanol) and fractions of A. speciosum (n-Hexane, n- Hexane: DCM, and DCM) have shown
enhancing effect in wound healing. These results strongly suggested the beneficial effects of both plant extracts for wound healing treatment and thus justified their medicinal use. |
| first_indexed | 2025-11-14T15:23:48Z |
| format | Proceeding Paper |
| id | iium-27426 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:23:48Z |
| publishDate | 2012 |
| publisher | Ibnu Sina Institute, UTM |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-274262017-06-15T06:12:20Z http://irep.iium.edu.my/27426/ In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd Herman, Hendy Putra Darnis, Deny Susanti Saad, Shahbudin Bakhtiar, M. Taher Ramli, Norazsida RS Pharmacy and materia medica Medicinal use of Acrostichum aureum and A. speciosum in wound healing treatment by Malay community was tested scientifically in this project. Wound healing properties of ethanolic, aqueous extracts and the fractions of A. aureum and A. speciosum were evaluated in vitro using scratch wound assay on NIH/3T3 cell line. In total, 22 different extracts and fractions from leaves, stems, and rhizomes of both species were used for this testing. NIH/ 3T3 cell lines were seeded at a density of 5 x 104 cells/ mL in 24-well plate and incubated at 37°C until reaching nearly confluence. The confluent cell monolayer was manually scratched with a sterile pipette tip. This test was done in triplicate. For the treatment, each well was treated with 0.2 mg/ mL of the different extracts, 2 ng/ mL of platelet derived growth factor (PDGF) as positive control and DMEM medium as negative control. The images before treatment and after 12 hour post-treatment were photographed using ProgRes® CapturePro 2.5 software to measure mean migration rate (μm/hour) and open image area (%) using TScratch software. The results showed that cell line treated with aqueous extract of rhizomes and stems of both species have improved the migration rate. The fractions of A. aureum (n-Hexane, n- Hexane: DCM, and ethanol) and fractions of A. speciosum (n-Hexane, n- Hexane: DCM, and DCM) have shown enhancing effect in wound healing. These results strongly suggested the beneficial effects of both plant extracts for wound healing treatment and thus justified their medicinal use. Ibnu Sina Institute, UTM 2012 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/27426/1/ISPC_2012_PROCEEDING_BOOK.pdf Herman, Hendy Putra and Darnis, Deny Susanti and Saad, Shahbudin and Bakhtiar, M. Taher and Ramli, Norazsida (2012) In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd. In: International Science Postgraduate Conference 2012 (ISPC 2012), 27-30 November 2012, Skudai, Johor. http://www.fs.utm.my/ispc2012/index.php/welcoming-message-nspc |
| spellingShingle | RS Pharmacy and materia medica Herman, Hendy Putra Darnis, Deny Susanti Saad, Shahbudin Bakhtiar, M. Taher Ramli, Norazsida In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd |
| title | In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd |
| title_full | In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd |
| title_fullStr | In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd |
| title_full_unstemmed | In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd |
| title_short | In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd |
| title_sort | in vitro evaluation of wound healing properties of fractions and extracts of acrostichum aureum linné and a. speciosum willd |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/27426/ http://irep.iium.edu.my/27426/ http://irep.iium.edu.my/27426/1/ISPC_2012_PROCEEDING_BOOK.pdf |