Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds

The effect of homogenization in breaking protein-carotenoid complexes from cassava leaves was examined by monitoring three process conditions; amount of solvent per 0.5 g sample (ml), homogenization speed (rpm) and homogenization time (s). Acetone-n-hexane (1:1, v/v), tetrahydrofuran-methanol (1:...

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Main Authors: Jamal, Parveen, Jaswir, Irwandi, Hashim, Nurhasri Mulyadi, Azmi, Saiful Mohammad Nizam
Format: Book Chapter
Language:English
Published: IIUM Press 2011
Subjects:
Online Access:http://irep.iium.edu.my/20992/
http://irep.iium.edu.my/20992/1/effect_of_homogenization_-_C36.pdf
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author Jamal, Parveen
Jaswir, Irwandi
Hashim, Nurhasri Mulyadi
Azmi, Saiful Mohammad Nizam
author_facet Jamal, Parveen
Jaswir, Irwandi
Hashim, Nurhasri Mulyadi
Azmi, Saiful Mohammad Nizam
author_sort Jamal, Parveen
building IIUM Repository
collection Online Access
description The effect of homogenization in breaking protein-carotenoid complexes from cassava leaves was examined by monitoring three process conditions; amount of solvent per 0.5 g sample (ml), homogenization speed (rpm) and homogenization time (s). Acetone-n-hexane (1:1, v/v), tetrahydrofuran-methanol (1:1 v/v) and acetone were employed in the extraction process. The solvent that exhibited the best characteristics in extracting the highest b-carotene yield from the sample was selected for the optimizatIOn of process conditions for maximum b-carotene concentration, utilizing Face Centered Central Composite Design (FCCD) from Design Expert software, version 6 (DX6). A total of 20 experiments comprised of three factors, three levels and 6 center points were conducted to facilitate the optimization process. The maximum b-carotene yield of 17,544 flg/lOO g sample was achieved with the solvent ratio of 12 ml to 0.5 g sample, homogenization speed of 22000 rpm and homogemzation time of 57 s.
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institution International Islamic University Malaysia
institution_category Local University
language English
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publishDate 2011
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spelling iium-209922021-06-01T05:35:07Z http://irep.iium.edu.my/20992/ Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds Jamal, Parveen Jaswir, Irwandi Hashim, Nurhasri Mulyadi Azmi, Saiful Mohammad Nizam TP248.13 Biotechnology The effect of homogenization in breaking protein-carotenoid complexes from cassava leaves was examined by monitoring three process conditions; amount of solvent per 0.5 g sample (ml), homogenization speed (rpm) and homogenization time (s). Acetone-n-hexane (1:1, v/v), tetrahydrofuran-methanol (1:1 v/v) and acetone were employed in the extraction process. The solvent that exhibited the best characteristics in extracting the highest b-carotene yield from the sample was selected for the optimizatIOn of process conditions for maximum b-carotene concentration, utilizing Face Centered Central Composite Design (FCCD) from Design Expert software, version 6 (DX6). A total of 20 experiments comprised of three factors, three levels and 6 center points were conducted to facilitate the optimization process. The maximum b-carotene yield of 17,544 flg/lOO g sample was achieved with the solvent ratio of 12 ml to 0.5 g sample, homogenization speed of 22000 rpm and homogemzation time of 57 s. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20992/1/effect_of_homogenization_-_C36.pdf Jamal, Parveen and Jaswir, Irwandi and Hashim, Nurhasri Mulyadi and Azmi, Saiful Mohammad Nizam (2011) Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 285-295. ISBN 9789674181444
spellingShingle TP248.13 Biotechnology
Jamal, Parveen
Jaswir, Irwandi
Hashim, Nurhasri Mulyadi
Azmi, Saiful Mohammad Nizam
Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
title Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
title_full Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
title_fullStr Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
title_full_unstemmed Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
title_short Effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
title_sort effect of homogenization in breaking protein-carotenoid complexes for releasing active compounds
topic TP248.13 Biotechnology
url http://irep.iium.edu.my/20992/
http://irep.iium.edu.my/20992/1/effect_of_homogenization_-_C36.pdf