Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter
Pharmaceuticals produced in the latex cytosol of genetically transformed Hevea can be harvested nondestructively by conventional tapping. This study evaluates the promoter activity of the 5’-untranslated upstream regulating region of hevein gene, which encodes the most abundant soluble protein in H...
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| Format: | Proceeding Paper |
| Language: | English |
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2010
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| Online Access: | http://irep.iium.edu.my/15900/ http://irep.iium.edu.my/15900/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf |
| _version_ | 1848777853856907264 |
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| author | Pappusamy, Arokiaraj Shuib, Siti Shuhada Badaruddin, Badrul Ezam Sunderasan, E. |
| author_facet | Pappusamy, Arokiaraj Shuib, Siti Shuhada Badaruddin, Badrul Ezam Sunderasan, E. |
| author_sort | Pappusamy, Arokiaraj |
| building | IIUM Repository |
| collection | Online Access |
| description | Pharmaceuticals produced in the latex cytosol of genetically transformed Hevea can be harvested nondestructively by conventional tapping. This study evaluates the promoter activity of the 5’-untranslated
upstream regulating region of hevein gene, which encodes the most abundant soluble protein in Hevea latex. Constructs were prepared with the test hevein promoter fragments Hev P1 (0.35kb), Hev P2 (0.45kb) and Hev P3 (0.73kb) that were inserted 5’ to the pharmaceutical genes i.e. human protamine 1 (HP1) and human atrial natriur tic factor (HANF), in pGPTV-Kan expression vector. The expression vectors containing HP1 and HANF were electroporated into Agrobacterium tumefaciens GV2260
containing supervirulent plasmid pToK47, which were then used to infect Hevea anther callus. The growth of the putative transformed Hevea callus was monitored on kanamycin selection media. The presence of the pharmaceutical genes (HP1 and HANF), the hevein promoter fragments, and nptII selection marker were verified by PCR on sampled putative transformed callus. The remaining callus tissues will be sub-cultured and transferred into differentiation media for embryoids formation and plantlet regeneration. |
| first_indexed | 2025-11-14T14:52:35Z |
| format | Proceeding Paper |
| id | iium-15900 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:52:35Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-159002012-01-30T00:06:48Z http://irep.iium.edu.my/15900/ Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter Pappusamy, Arokiaraj Shuib, Siti Shuhada Badaruddin, Badrul Ezam Sunderasan, E. TP248.13 Biotechnology Pharmaceuticals produced in the latex cytosol of genetically transformed Hevea can be harvested nondestructively by conventional tapping. This study evaluates the promoter activity of the 5’-untranslated upstream regulating region of hevein gene, which encodes the most abundant soluble protein in Hevea latex. Constructs were prepared with the test hevein promoter fragments Hev P1 (0.35kb), Hev P2 (0.45kb) and Hev P3 (0.73kb) that were inserted 5’ to the pharmaceutical genes i.e. human protamine 1 (HP1) and human atrial natriur tic factor (HANF), in pGPTV-Kan expression vector. The expression vectors containing HP1 and HANF were electroporated into Agrobacterium tumefaciens GV2260 containing supervirulent plasmid pToK47, which were then used to infect Hevea anther callus. The growth of the putative transformed Hevea callus was monitored on kanamycin selection media. The presence of the pharmaceutical genes (HP1 and HANF), the hevein promoter fragments, and nptII selection marker were verified by PCR on sampled putative transformed callus. The remaining callus tissues will be sub-cultured and transferred into differentiation media for embryoids formation and plantlet regeneration. 2010 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/15900/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf Pappusamy, Arokiaraj and Shuib, Siti Shuhada and Badaruddin, Badrul Ezam and Sunderasan, E. (2010) Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. http://www.iium.edu.my/irie/10/info/Programme_Book%20Part_2.pdf |
| spellingShingle | TP248.13 Biotechnology Pappusamy, Arokiaraj Shuib, Siti Shuhada Badaruddin, Badrul Ezam Sunderasan, E. Hevea genetic transformation for enhanced recombinant pharmaceutical production by the use of hevein promoter |
| title | Hevea genetic transformation for enhanced recombinant
pharmaceutical production by the use of hevein promoter |
| title_full | Hevea genetic transformation for enhanced recombinant
pharmaceutical production by the use of hevein promoter |
| title_fullStr | Hevea genetic transformation for enhanced recombinant
pharmaceutical production by the use of hevein promoter |
| title_full_unstemmed | Hevea genetic transformation for enhanced recombinant
pharmaceutical production by the use of hevein promoter |
| title_short | Hevea genetic transformation for enhanced recombinant
pharmaceutical production by the use of hevein promoter |
| title_sort | hevea genetic transformation for enhanced recombinant
pharmaceutical production by the use of hevein promoter |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/15900/ http://irep.iium.edu.my/15900/ http://irep.iium.edu.my/15900/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf |