Facile synthesis and antimycobacterial activity of isoniazid, pyrazinamide and ciprofloxacin derivatives

Several rationally designed isoniazid (INH), pyrazinamide (PZA) and ciprofloxacin (CPF) derivatives were conveniently synthesized and evaluated in vitro against H37Rv Mycobacterium tuberculosis (M. tb) strain. CPF derivative 16 displayed a modest activity (MIC = 16 µg/ml) and was docked into the M....

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Main Authors: Alsayed, Shahinda S.R., Lun, S., Payne, Alan, Bishai, William R., Gunosewoyo, Hendra
Format: Journal Article
Language:English
Published: WILEY 2021
Subjects:
Online Access:http://purl.org/au-research/grants/arc/DE160100482
http://hdl.handle.net/20.500.11937/90990
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author Alsayed, Shahinda S.R.
Lun, S.
Payne, Alan
Bishai, William R.
Gunosewoyo, Hendra
author_facet Alsayed, Shahinda S.R.
Lun, S.
Payne, Alan
Bishai, William R.
Gunosewoyo, Hendra
author_sort Alsayed, Shahinda S.R.
building Curtin Institutional Repository
collection Online Access
description Several rationally designed isoniazid (INH), pyrazinamide (PZA) and ciprofloxacin (CPF) derivatives were conveniently synthesized and evaluated in vitro against H37Rv Mycobacterium tuberculosis (M. tb) strain. CPF derivative 16 displayed a modest activity (MIC = 16 µg/ml) and was docked into the M. tb DNA gyrase. Isoniazid-pyrazinoic acid (INH-POA) hybrid 21a showed the highest potency in our study (MIC = 2 µg/ml). It also retained its high activity against the other tested M. tb drug-sensitive strain (DS) V4207 (MIC = 4 µg/ml) and demonstrated negligible cytotoxicity against Vero cells (IC50 ≥ 64 µg/ml). Four tested drug-resistant (DR) M. tb strains were refractory to 21a, similar to INH, whilst being sensitive to CPF. Compound 21a was also inactive against two non-tuberculous mycobacterial (NTM) strains, suggesting its selective activity against M. tb. The noteworthy activity of 21a against DS strains and its low cytotoxicity highlight its potential to treat DS M. tb.
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spelling curtin-20.500.11937-909902023-05-24T01:28:51Z Facile synthesis and antimycobacterial activity of isoniazid, pyrazinamide and ciprofloxacin derivatives Alsayed, Shahinda S.R. Lun, S. Payne, Alan Bishai, William R. Gunosewoyo, Hendra Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Chemistry, Medicinal Pharmacology & Pharmacy ciprofloxacin hybrid molecules indoleamides isoniazid pyrazinamide tuberculosis ciprofloxacin hybrid molecules indoleamides isoniazid pyrazinamide tuberculosis Animals Antitubercular Agents Binding Sites Catalytic Domain Cell Survival Chlorocebus aethiops Ciprofloxacin DNA Gyrase Drug Design Drug Resistance, Bacterial Isoniazid Microbial Sensitivity Tests Molecular Conformation Molecular Docking Simulation Mycobacterium tuberculosis Nontuberculous Mycobacteria Pyrazinamide Structure-Activity Relationship Vero Cells Vero Cells Animals Mycobacterium tuberculosis Isoniazid Pyrazinamide Ciprofloxacin DNA Gyrase Antitubercular Agents Microbial Sensitivity Tests Drug Resistance, Bacterial Cell Survival Binding Sites Catalytic Domain Molecular Conformation Structure-Activity Relationship Drug Design Nontuberculous Mycobacteria Molecular Docking Simulation Chlorocebus aethiops Several rationally designed isoniazid (INH), pyrazinamide (PZA) and ciprofloxacin (CPF) derivatives were conveniently synthesized and evaluated in vitro against H37Rv Mycobacterium tuberculosis (M. tb) strain. CPF derivative 16 displayed a modest activity (MIC = 16 µg/ml) and was docked into the M. tb DNA gyrase. Isoniazid-pyrazinoic acid (INH-POA) hybrid 21a showed the highest potency in our study (MIC = 2 µg/ml). It also retained its high activity against the other tested M. tb drug-sensitive strain (DS) V4207 (MIC = 4 µg/ml) and demonstrated negligible cytotoxicity against Vero cells (IC50 ≥ 64 µg/ml). Four tested drug-resistant (DR) M. tb strains were refractory to 21a, similar to INH, whilst being sensitive to CPF. Compound 21a was also inactive against two non-tuberculous mycobacterial (NTM) strains, suggesting its selective activity against M. tb. The noteworthy activity of 21a against DS strains and its low cytotoxicity highlight its potential to treat DS M. tb. 2021 Journal Article http://hdl.handle.net/20.500.11937/90990 10.1111/cbdd.13836 English http://purl.org/au-research/grants/arc/DE160100482 WILEY fulltext
spellingShingle Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Chemistry, Medicinal
Pharmacology & Pharmacy
ciprofloxacin
hybrid molecules
indoleamides
isoniazid
pyrazinamide
tuberculosis
ciprofloxacin
hybrid molecules
indoleamides
isoniazid
pyrazinamide
tuberculosis
Animals
Antitubercular Agents
Binding Sites
Catalytic Domain
Cell Survival
Chlorocebus aethiops
Ciprofloxacin
DNA Gyrase
Drug Design
Drug Resistance, Bacterial
Isoniazid
Microbial Sensitivity Tests
Molecular Conformation
Molecular Docking Simulation
Mycobacterium tuberculosis
Nontuberculous Mycobacteria
Pyrazinamide
Structure-Activity Relationship
Vero Cells
Vero Cells
Animals
Mycobacterium tuberculosis
Isoniazid
Pyrazinamide
Ciprofloxacin
DNA Gyrase
Antitubercular Agents
Microbial Sensitivity Tests
Drug Resistance, Bacterial
Cell Survival
Binding Sites
Catalytic Domain
Molecular Conformation
Structure-Activity Relationship
Drug Design
Nontuberculous Mycobacteria
Molecular Docking Simulation
Chlorocebus aethiops
Alsayed, Shahinda S.R.
Lun, S.
Payne, Alan
Bishai, William R.
Gunosewoyo, Hendra
Facile synthesis and antimycobacterial activity of isoniazid, pyrazinamide and ciprofloxacin derivatives
title Facile synthesis and antimycobacterial activity of isoniazid, pyrazinamide and ciprofloxacin derivatives
title_full Facile synthesis and antimycobacterial activity of isoniazid, pyrazinamide and ciprofloxacin derivatives
title_fullStr Facile synthesis and antimycobacterial activity of isoniazid, pyrazinamide and ciprofloxacin derivatives
title_full_unstemmed Facile synthesis and antimycobacterial activity of isoniazid, pyrazinamide and ciprofloxacin derivatives
title_short Facile synthesis and antimycobacterial activity of isoniazid, pyrazinamide and ciprofloxacin derivatives
title_sort facile synthesis and antimycobacterial activity of isoniazid, pyrazinamide and ciprofloxacin derivatives
topic Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Chemistry, Medicinal
Pharmacology & Pharmacy
ciprofloxacin
hybrid molecules
indoleamides
isoniazid
pyrazinamide
tuberculosis
ciprofloxacin
hybrid molecules
indoleamides
isoniazid
pyrazinamide
tuberculosis
Animals
Antitubercular Agents
Binding Sites
Catalytic Domain
Cell Survival
Chlorocebus aethiops
Ciprofloxacin
DNA Gyrase
Drug Design
Drug Resistance, Bacterial
Isoniazid
Microbial Sensitivity Tests
Molecular Conformation
Molecular Docking Simulation
Mycobacterium tuberculosis
Nontuberculous Mycobacteria
Pyrazinamide
Structure-Activity Relationship
Vero Cells
Vero Cells
Animals
Mycobacterium tuberculosis
Isoniazid
Pyrazinamide
Ciprofloxacin
DNA Gyrase
Antitubercular Agents
Microbial Sensitivity Tests
Drug Resistance, Bacterial
Cell Survival
Binding Sites
Catalytic Domain
Molecular Conformation
Structure-Activity Relationship
Drug Design
Nontuberculous Mycobacteria
Molecular Docking Simulation
Chlorocebus aethiops
url http://purl.org/au-research/grants/arc/DE160100482
http://hdl.handle.net/20.500.11937/90990