Antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds
The glucose modulating properties of lupin have been attributed to its seed protein γ-conglutin. Here we explored the antidiabetic potential of γ-conglutin purified from lupin seeds in-vitro. To mimic the effects of an orally administered supplement, purified γ-conglutin was hydrolysed by gastrointe...
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| Format: | Journal Article |
| Language: | English |
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ELSEVIER
2021
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| Online Access: | http://purl.org/au-research/grants/arc/LP190100130 http://hdl.handle.net/20.500.11937/90173 |
| _version_ | 1848765345022607360 |
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| author | Tapadia, Mrunmai Johnson, Stuart Utikar, Ranjeet Newsholme, Philip Carlessi, Rodrigo |
| author_facet | Tapadia, Mrunmai Johnson, Stuart Utikar, Ranjeet Newsholme, Philip Carlessi, Rodrigo |
| author_sort | Tapadia, Mrunmai |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The glucose modulating properties of lupin have been attributed to its seed protein γ-conglutin. Here we explored the antidiabetic potential of γ-conglutin purified from lupin seeds in-vitro. To mimic the effects of an orally administered supplement, purified γ-conglutin was hydrolysed by gastrointestinal proteolytic enzymes and the resulting peptides evaluated for their antidiabetic effects in pancreatic β-cells and primary human skeletal muscle myotubes. γ-conglutin peptides did not promote insulin secretion in β-cells but elicited a potent insulin-mimetic action by activating insulin signalling pathways responsible for glycogen, protein synthesis, and glucose transport into myotubes. Additionally, the peptides potently suppressed the activity of DPP4 indicating their potential to increase the half-life of incretin hormones in circulation. These results substantiate the health benefits of consuming lupin seeds as part of a healthy diet and can drive the current market for lupins from primarily stockfeed, towards value-added lupin-based food products for human consumption. |
| first_indexed | 2025-11-14T11:33:46Z |
| format | Journal Article |
| id | curtin-20.500.11937-90173 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:33:46Z |
| publishDate | 2021 |
| publisher | ELSEVIER |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-901732023-02-20T04:09:38Z Antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds Tapadia, Mrunmai Johnson, Stuart Utikar, Ranjeet Newsholme, Philip Carlessi, Rodrigo Science & Technology Life Sciences & Biomedicine Food Science & Technology Nutrition & Dietetics Lupin seeds gamma-conglutin Antidiabetic Insulin mimetic DPP4 inhibitor GLUCAGON-LIKE PEPTIDE-1 WHEY-PROTEIN DIPEPTIDYL PEPTIDASE-4 POSTPRANDIAL GLUCOSE ALPHA-GLUCOSIDASE FUNCTIONAL FOODS LOWERING PROTEIN BLOOD-GLUCOSE IN-VITRO INSULIN The glucose modulating properties of lupin have been attributed to its seed protein γ-conglutin. Here we explored the antidiabetic potential of γ-conglutin purified from lupin seeds in-vitro. To mimic the effects of an orally administered supplement, purified γ-conglutin was hydrolysed by gastrointestinal proteolytic enzymes and the resulting peptides evaluated for their antidiabetic effects in pancreatic β-cells and primary human skeletal muscle myotubes. γ-conglutin peptides did not promote insulin secretion in β-cells but elicited a potent insulin-mimetic action by activating insulin signalling pathways responsible for glycogen, protein synthesis, and glucose transport into myotubes. Additionally, the peptides potently suppressed the activity of DPP4 indicating their potential to increase the half-life of incretin hormones in circulation. These results substantiate the health benefits of consuming lupin seeds as part of a healthy diet and can drive the current market for lupins from primarily stockfeed, towards value-added lupin-based food products for human consumption. 2021 Journal Article http://hdl.handle.net/20.500.11937/90173 10.1016/j.jff.2021.104786 English http://purl.org/au-research/grants/arc/LP190100130 http://creativecommons.org/licenses/by/4.0/ ELSEVIER fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Food Science & Technology Nutrition & Dietetics Lupin seeds gamma-conglutin Antidiabetic Insulin mimetic DPP4 inhibitor GLUCAGON-LIKE PEPTIDE-1 WHEY-PROTEIN DIPEPTIDYL PEPTIDASE-4 POSTPRANDIAL GLUCOSE ALPHA-GLUCOSIDASE FUNCTIONAL FOODS LOWERING PROTEIN BLOOD-GLUCOSE IN-VITRO INSULIN Tapadia, Mrunmai Johnson, Stuart Utikar, Ranjeet Newsholme, Philip Carlessi, Rodrigo Antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds |
| title | Antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds |
| title_full | Antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds |
| title_fullStr | Antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds |
| title_full_unstemmed | Antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds |
| title_short | Antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds |
| title_sort | antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds |
| topic | Science & Technology Life Sciences & Biomedicine Food Science & Technology Nutrition & Dietetics Lupin seeds gamma-conglutin Antidiabetic Insulin mimetic DPP4 inhibitor GLUCAGON-LIKE PEPTIDE-1 WHEY-PROTEIN DIPEPTIDYL PEPTIDASE-4 POSTPRANDIAL GLUCOSE ALPHA-GLUCOSIDASE FUNCTIONAL FOODS LOWERING PROTEIN BLOOD-GLUCOSE IN-VITRO INSULIN |
| url | http://purl.org/au-research/grants/arc/LP190100130 http://hdl.handle.net/20.500.11937/90173 |