Endotoxin removal and plasmid DNA recovery in various metal ion solutions
In this brief article, we report endotoxin precipitation using different types of metal ion in plasmid DNA purification. The work involved modification and improvement of the LAL chromogenic analysis to compensate for metal ion-LAL interaction. Results (Figs. 1 and 2) show that ZnSO4 gives an optimu...
| Main Authors: | , |
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| Format: | Journal Article |
| Published: |
Taylor and Francis
2011
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| Online Access: | http://www.tandfonline.com/doi/full/10.1080/01496395.2011.557027 http://hdl.handle.net/20.500.11937/8774 |
| _version_ | 1848745757271654400 |
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| author | Ongkudon, C. Danquah, Michael |
| author_facet | Ongkudon, C. Danquah, Michael |
| author_sort | Ongkudon, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In this brief article, we report endotoxin precipitation using different types of metal ion in plasmid DNA purification. The work involved modification and improvement of the LAL chromogenic analysis to compensate for metal ion-LAL interaction. Results (Figs. 1 and 2) show that ZnSO4 gives an optimum combination of high endotoxin removal efficiency (~91%) as well as high plasmid recovery (~100%) compared to other metal ions tested. |
| first_indexed | 2025-11-14T06:22:26Z |
| format | Journal Article |
| id | curtin-20.500.11937-8774 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:22:26Z |
| publishDate | 2011 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-87742019-02-19T04:26:34Z Endotoxin removal and plasmid DNA recovery in various metal ion solutions Ongkudon, C. Danquah, Michael In this brief article, we report endotoxin precipitation using different types of metal ion in plasmid DNA purification. The work involved modification and improvement of the LAL chromogenic analysis to compensate for metal ion-LAL interaction. Results (Figs. 1 and 2) show that ZnSO4 gives an optimum combination of high endotoxin removal efficiency (~91%) as well as high plasmid recovery (~100%) compared to other metal ions tested. 2011 Journal Article http://hdl.handle.net/20.500.11937/8774 10.1080/01496395.2011.557027 http://www.tandfonline.com/doi/full/10.1080/01496395.2011.557027 Taylor and Francis restricted |
| spellingShingle | Ongkudon, C. Danquah, Michael Endotoxin removal and plasmid DNA recovery in various metal ion solutions |
| title | Endotoxin removal and plasmid DNA recovery in various metal ion solutions |
| title_full | Endotoxin removal and plasmid DNA recovery in various metal ion solutions |
| title_fullStr | Endotoxin removal and plasmid DNA recovery in various metal ion solutions |
| title_full_unstemmed | Endotoxin removal and plasmid DNA recovery in various metal ion solutions |
| title_short | Endotoxin removal and plasmid DNA recovery in various metal ion solutions |
| title_sort | endotoxin removal and plasmid dna recovery in various metal ion solutions |
| url | http://www.tandfonline.com/doi/full/10.1080/01496395.2011.557027 http://hdl.handle.net/20.500.11937/8774 |