Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes

Lysine specific demethylase 1 (LSD1) is a histone modifying enzyme that suppresses gene expression through demethylation of lysine 4 on histone H3. The anti-tumor activity of GSK2879552 and GSK-LSD1, potent, selective irreversible inactivators of LSD1, has previously been described. Inhibition of LS...

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Main Authors: Smitheman, K.N., Severson, T.M., Rajapurkar, S.R., McCabe, M.T., Karpinich, N., Foley, J., Pappalardi, M.B., Hughes, A., Halsey, W., Thomas, E., Traini, C., Federowicz, K.E., Laraio, J., Mobegi, Fredrick, Ferron-Brady, G., Prinjha, R.K., Carpenter, C.L., Kruger, R.G., Wessels, L., Mohammad, H.P.
Format: Journal Article
Language:English
Published: FERRATA STORTI FOUNDATION 2019
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/80728
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author Smitheman, K.N.
Severson, T.M.
Rajapurkar, S.R.
McCabe, M.T.
Karpinich, N.
Foley, J.
Pappalardi, M.B.
Hughes, A.
Halsey, W.
Thomas, E.
Traini, C.
Federowicz, K.E.
Laraio, J.
Mobegi, Fredrick
Ferron-Brady, G.
Prinjha, R.K.
Carpenter, C.L.
Kruger, R.G.
Wessels, L.
Mohammad, H.P.
author_facet Smitheman, K.N.
Severson, T.M.
Rajapurkar, S.R.
McCabe, M.T.
Karpinich, N.
Foley, J.
Pappalardi, M.B.
Hughes, A.
Halsey, W.
Thomas, E.
Traini, C.
Federowicz, K.E.
Laraio, J.
Mobegi, Fredrick
Ferron-Brady, G.
Prinjha, R.K.
Carpenter, C.L.
Kruger, R.G.
Wessels, L.
Mohammad, H.P.
author_sort Smitheman, K.N.
building Curtin Institutional Repository
collection Online Access
description Lysine specific demethylase 1 (LSD1) is a histone modifying enzyme that suppresses gene expression through demethylation of lysine 4 on histone H3. The anti-tumor activity of GSK2879552 and GSK-LSD1, potent, selective irreversible inactivators of LSD1, has previously been described. Inhibition of LSD1 results in a cytostatic growth inhibitory effect in a range of acute myeloid leukemia cell lines. To enhance the therapeutic potential of LSD1 inhibition in this disease setting, a combination of LSD1 inhibition and all-trans retinoic acid was explored. All-trans retinoic acid is currently approved for use in acute promyelocytic leukemia in which it promotes differentiation of abnormal blast cells into normal white blood cells. Combined treatment with all-trans retinoic acid and GSK2879552 results in synergistic effects on cell proliferation, markers of differentiation, and, most importantly, cytotoxicity. Ultimately the combination potential for LSD1 inhibition and ATRA will require validation in acute myeloid leukemia patients, and clinical studies to assess this are currently underway.
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spelling curtin-20.500.11937-807282020-10-30T03:18:30Z Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes Smitheman, K.N. Severson, T.M. Rajapurkar, S.R. McCabe, M.T. Karpinich, N. Foley, J. Pappalardi, M.B. Hughes, A. Halsey, W. Thomas, E. Traini, C. Federowicz, K.E. Laraio, J. Mobegi, Fredrick Ferron-Brady, G. Prinjha, R.K. Carpenter, C.L. Kruger, R.G. Wessels, L. Mohammad, H.P. Science & Technology Life Sciences & Biomedicine Hematology HUMAN BONE-MARROW SUPEROXIDE ANION DISEASE INHIBITION NEUTROPHILS APOPTOSIS THERAPY Lysine specific demethylase 1 (LSD1) is a histone modifying enzyme that suppresses gene expression through demethylation of lysine 4 on histone H3. The anti-tumor activity of GSK2879552 and GSK-LSD1, potent, selective irreversible inactivators of LSD1, has previously been described. Inhibition of LSD1 results in a cytostatic growth inhibitory effect in a range of acute myeloid leukemia cell lines. To enhance the therapeutic potential of LSD1 inhibition in this disease setting, a combination of LSD1 inhibition and all-trans retinoic acid was explored. All-trans retinoic acid is currently approved for use in acute promyelocytic leukemia in which it promotes differentiation of abnormal blast cells into normal white blood cells. Combined treatment with all-trans retinoic acid and GSK2879552 results in synergistic effects on cell proliferation, markers of differentiation, and, most importantly, cytotoxicity. Ultimately the combination potential for LSD1 inhibition and ATRA will require validation in acute myeloid leukemia patients, and clinical studies to assess this are currently underway. 2019 Journal Article http://hdl.handle.net/20.500.11937/80728 10.3324/haematol.2018.199190 English http://creativecommons.org/licenses/by-nc/4.0/ FERRATA STORTI FOUNDATION fulltext
spellingShingle Science & Technology
Life Sciences & Biomedicine
Hematology
HUMAN BONE-MARROW
SUPEROXIDE ANION
DISEASE
INHIBITION
NEUTROPHILS
APOPTOSIS
THERAPY
Smitheman, K.N.
Severson, T.M.
Rajapurkar, S.R.
McCabe, M.T.
Karpinich, N.
Foley, J.
Pappalardi, M.B.
Hughes, A.
Halsey, W.
Thomas, E.
Traini, C.
Federowicz, K.E.
Laraio, J.
Mobegi, Fredrick
Ferron-Brady, G.
Prinjha, R.K.
Carpenter, C.L.
Kruger, R.G.
Wessels, L.
Mohammad, H.P.
Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes
title Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes
title_full Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes
title_fullStr Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes
title_full_unstemmed Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes
title_short Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes
title_sort lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes
topic Science & Technology
Life Sciences & Biomedicine
Hematology
HUMAN BONE-MARROW
SUPEROXIDE ANION
DISEASE
INHIBITION
NEUTROPHILS
APOPTOSIS
THERAPY
url http://hdl.handle.net/20.500.11937/80728