Exogenous dihydrosphingosine 1 phosphate mediates collagen synthesis in cardiac fibroblasts through JAK/STAT signalling and regulation of TIMP1
© 2020 Elsevier Inc. Cardiac fibrosis and myocyte hypertrophy are hallmarks of the cardiac remodelling process in cardiomyopathies such as heart failure (HF). Dyslipidemia or dysregulation of lipids contribute to HF. The dysregulation of high density lipoproteins (HDL) could lead to altered leve...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2020
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| Online Access: | http://purl.org/au-research/grants/nhmrc/1092642 http://hdl.handle.net/20.500.11937/80042 |
| _version_ | 1848764146895552512 |
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| author | Magaye, R.R. Savira, F. Hua, Y. Xiong, X. Huang, L. Reid, Christopher Flynn, B. Kaye, D. Liew, D. Wang, B.H. |
| author_facet | Magaye, R.R. Savira, F. Hua, Y. Xiong, X. Huang, L. Reid, Christopher Flynn, B. Kaye, D. Liew, D. Wang, B.H. |
| author_sort | Magaye, R.R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2020 Elsevier Inc.
Cardiac fibrosis and myocyte hypertrophy are hallmarks of the cardiac remodelling process in cardiomyopathies such as heart failure (HF). Dyslipidemia or dysregulation of lipids contribute to HF. The dysregulation of high density lipoproteins (HDL) could lead to altered levels of other lipid metabolites that are bound to it such as sphingosine-1- phosphate (S1P). Recently, it has been shown that S1P and its analogue dihydrosphingosine-1-phosphate (dhS1P) are bound to HDL in plasma. The effects of dhS1P on cardiac cells have been obscure. In this study, we show that extracellular dhS1P is able to increase collagen synthesis in neonatal rat cardiac fibroblasts (NCFs) and cause hypertrophy of neonatal cardiac myocytes (NCMs). The janus kinase/signal transducer and activator (JAK/STAT) signalling pathway was involved in the increased collagen synthesis by dhS1P, through sustained increase of tissue inhibitor of matrix metalloproteinase 1 (TIMP1). Extracellular dhS1P increased phosphorylation levels of STAT1 and STAT3 proteins, also caused an early increase in gene expression of transforming growth factor-β (TGFβ), and sustained increase in TIMP1. Inhibition of JAKs led to inhibition of TIMP1 and TGFβ gene and protein expression. We also show that dhS1P is able to cause NCM hypertrophy through S1P-receptor-1 (S1PR1) signalling which is opposite to that of its analogue, S1P. Taken together, our results show that dhS1P increases collagen synthesis in cardiac fibroblasts causing fibrosis through dhS1P-JAK/STAT-TIMP1 signalling. |
| first_indexed | 2025-11-14T11:14:43Z |
| format | Journal Article |
| id | curtin-20.500.11937-80042 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | eng |
| last_indexed | 2025-11-14T11:14:43Z |
| publishDate | 2020 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-800422020-08-12T05:22:29Z Exogenous dihydrosphingosine 1 phosphate mediates collagen synthesis in cardiac fibroblasts through JAK/STAT signalling and regulation of TIMP1 Magaye, R.R. Savira, F. Hua, Y. Xiong, X. Huang, L. Reid, Christopher Flynn, B. Kaye, D. Liew, D. Wang, B.H. Cardiac remodelling Dihydropshingosine 1 phosphate JAK/STAT signalling Sphingolipid TIMP1 © 2020 Elsevier Inc. Cardiac fibrosis and myocyte hypertrophy are hallmarks of the cardiac remodelling process in cardiomyopathies such as heart failure (HF). Dyslipidemia or dysregulation of lipids contribute to HF. The dysregulation of high density lipoproteins (HDL) could lead to altered levels of other lipid metabolites that are bound to it such as sphingosine-1- phosphate (S1P). Recently, it has been shown that S1P and its analogue dihydrosphingosine-1-phosphate (dhS1P) are bound to HDL in plasma. The effects of dhS1P on cardiac cells have been obscure. In this study, we show that extracellular dhS1P is able to increase collagen synthesis in neonatal rat cardiac fibroblasts (NCFs) and cause hypertrophy of neonatal cardiac myocytes (NCMs). The janus kinase/signal transducer and activator (JAK/STAT) signalling pathway was involved in the increased collagen synthesis by dhS1P, through sustained increase of tissue inhibitor of matrix metalloproteinase 1 (TIMP1). Extracellular dhS1P increased phosphorylation levels of STAT1 and STAT3 proteins, also caused an early increase in gene expression of transforming growth factor-β (TGFβ), and sustained increase in TIMP1. Inhibition of JAKs led to inhibition of TIMP1 and TGFβ gene and protein expression. We also show that dhS1P is able to cause NCM hypertrophy through S1P-receptor-1 (S1PR1) signalling which is opposite to that of its analogue, S1P. Taken together, our results show that dhS1P increases collagen synthesis in cardiac fibroblasts causing fibrosis through dhS1P-JAK/STAT-TIMP1 signalling. 2020 Journal Article http://hdl.handle.net/20.500.11937/80042 10.1016/j.cellsig.2020.109629 eng http://purl.org/au-research/grants/nhmrc/1092642 http://purl.org/au-research/grants/nhmrc/1087355 restricted |
| spellingShingle | Cardiac remodelling Dihydropshingosine 1 phosphate JAK/STAT signalling Sphingolipid TIMP1 Magaye, R.R. Savira, F. Hua, Y. Xiong, X. Huang, L. Reid, Christopher Flynn, B. Kaye, D. Liew, D. Wang, B.H. Exogenous dihydrosphingosine 1 phosphate mediates collagen synthesis in cardiac fibroblasts through JAK/STAT signalling and regulation of TIMP1 |
| title | Exogenous dihydrosphingosine 1 phosphate mediates collagen synthesis in cardiac fibroblasts through JAK/STAT signalling and regulation of TIMP1 |
| title_full | Exogenous dihydrosphingosine 1 phosphate mediates collagen synthesis in cardiac fibroblasts through JAK/STAT signalling and regulation of TIMP1 |
| title_fullStr | Exogenous dihydrosphingosine 1 phosphate mediates collagen synthesis in cardiac fibroblasts through JAK/STAT signalling and regulation of TIMP1 |
| title_full_unstemmed | Exogenous dihydrosphingosine 1 phosphate mediates collagen synthesis in cardiac fibroblasts through JAK/STAT signalling and regulation of TIMP1 |
| title_short | Exogenous dihydrosphingosine 1 phosphate mediates collagen synthesis in cardiac fibroblasts through JAK/STAT signalling and regulation of TIMP1 |
| title_sort | exogenous dihydrosphingosine 1 phosphate mediates collagen synthesis in cardiac fibroblasts through jak/stat signalling and regulation of timp1 |
| topic | Cardiac remodelling Dihydropshingosine 1 phosphate JAK/STAT signalling Sphingolipid TIMP1 |
| url | http://purl.org/au-research/grants/nhmrc/1092642 http://purl.org/au-research/grants/nhmrc/1092642 http://hdl.handle.net/20.500.11937/80042 |