A modified yeast three-hybrid system enabling both positive and negative selections

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A modified yeast three-hybrid system enabling both positive and negative selections

Objective: To increase the reporter repertoire of the yeast three-hybrid system and introduce the option of negative selection. Results: Two new versions of the yeast three-hybrid system were made by modifying the MS2 coat RNA-binding protein and fusing it to the Gal4 DNA-binding protein. This allow...

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Bibliographic Details
Main Authors:	Wallis, C.P., Filipovska, A., Rackham, Oliver
Format:	Journal Article
Language:	English
Published:	SPRINGER 2018
Subjects:	Science & Technology Life Sciences & Biomedicine Biotechnology & Applied Microbiology Genetic selection RNA-binding protein RNA-protein interactions Yeast three-hybrid system RNA-PROTEIN INTERACTIONS BINDING SPECIFICITY 2-HYBRID AFFINITY
Online Access:	http://purl.org/au-research/grants/arc/DP180101656 http://hdl.handle.net/20.500.11937/79273

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