A modified yeast three-hybrid system enabling both positive and negative selections

A modified yeast three-hybrid system enabling both positive and negative selections

Objective: To increase the reporter repertoire of the yeast three-hybrid system and introduce the option of negative selection. Results: Two new versions of the yeast three-hybrid system were made by modifying the MS2 coat RNA-binding protein and fusing it to the Gal4 DNA-binding protein. This allow...

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Bibliographic Details
Main Authors: Wallis, C.P., Filipovska, A., Rackham, Oliver
Format: Journal Article
Language:English
Published: SPRINGER 2018
Subjects:
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Genetic selection
RNA-binding protein
RNA-protein interactions
Yeast three-hybrid system
RNA-PROTEIN INTERACTIONS
BINDING
SPECIFICITY
2-HYBRID
AFFINITY
Online Access:http://purl.org/au-research/grants/arc/DP180101656
http://hdl.handle.net/20.500.11937/79273

Internet

http://purl.org/au-research/grants/arc/DP180101656
http://hdl.handle.net/20.500.11937/79273

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