Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene
The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | English |
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AMER SOC MICROBIOLOGY
2011
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/79137 |
| _version_ | 1848764005288509440 |
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| author | Christophersen, Claus Morrison, M. Conlon, M.A. |
| author_facet | Christophersen, Claus Morrison, M. Conlon, M.A. |
| author_sort | Christophersen, Claus |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting two genes involved in SRB energy metabolism. © 2011, American Society for Microbiology. All Rights Reserved. |
| first_indexed | 2025-11-14T11:12:28Z |
| format | Journal Article |
| id | curtin-20.500.11937-79137 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:12:28Z |
| publishDate | 2011 |
| publisher | AMER SOC MICROBIOLOGY |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-791372020-08-05T07:30:18Z Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene Christophersen, Claus Morrison, M. Conlon, M.A. Science & Technology Life Sciences & Biomedicine Biotechnology & Applied Microbiology Microbiology POLYMERASE-CHAIN-REACTION REAL-TIME PCR ULCERATIVE-COLITIS GASTROINTESTINAL-TRACT HYDROGEN-SULFIDE COLORECTAL-CANCER HEALTHY QUANTIFICATION IDENTIFICATION POPULATIONS The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting two genes involved in SRB energy metabolism. © 2011, American Society for Microbiology. All Rights Reserved. 2011 Journal Article http://hdl.handle.net/20.500.11937/79137 10.1128/AEM.02851-10 English AMER SOC MICROBIOLOGY restricted |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Biotechnology & Applied Microbiology Microbiology POLYMERASE-CHAIN-REACTION REAL-TIME PCR ULCERATIVE-COLITIS GASTROINTESTINAL-TRACT HYDROGEN-SULFIDE COLORECTAL-CANCER HEALTHY QUANTIFICATION IDENTIFICATION POPULATIONS Christophersen, Claus Morrison, M. Conlon, M.A. Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene |
| title | Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene |
| title_full | Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene |
| title_fullStr | Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene |
| title_full_unstemmed | Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene |
| title_short | Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene |
| title_sort | overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative pcr targeting the desulfovibrio 16s rrna gene |
| topic | Science & Technology Life Sciences & Biomedicine Biotechnology & Applied Microbiology Microbiology POLYMERASE-CHAIN-REACTION REAL-TIME PCR ULCERATIVE-COLITIS GASTROINTESTINAL-TRACT HYDROGEN-SULFIDE COLORECTAL-CANCER HEALTHY QUANTIFICATION IDENTIFICATION POPULATIONS |
| url | http://hdl.handle.net/20.500.11937/79137 |