Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene

Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene

The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting...

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Bibliographic Details
Main Authors: Christophersen, Claus, Morrison, M., Conlon, M.A.
Format: Journal Article
Language:English
Published: AMER SOC MICROBIOLOGY 2011
Subjects:
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Microbiology
POLYMERASE-CHAIN-REACTION
REAL-TIME PCR
ULCERATIVE-COLITIS
GASTROINTESTINAL-TRACT
HYDROGEN-SULFIDE
COLORECTAL-CANCER
HEALTHY
QUANTIFICATION
IDENTIFICATION
POPULATIONS
Online Access:http://hdl.handle.net/20.500.11937/79137

Internet

http://hdl.handle.net/20.500.11937/79137

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