Selection of housekeeping genes for real-time PCR in atopic human bronchial epithelial cells

Selection of housekeeping genes for real-time PCR in atopic human bronchial epithelial cells

The stability of housekeeping genes (HKGs) is critical when performing real-time quantitative PCR. To date, the stability of common HKGs has not been systematically compared in human airway epithelial cells (AEC) in normal and atopic subjects. Expression levels of 12 HKGs were measured in AECs from...

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Bibliographic Details
Main Authors: He, J.Q., Sandford, A.J., Wang, I.M., Stepaniants, S., Knight, D.A., Kicic, Anthony, Stick, S.M., Paré, P.D.
Format: Journal Article
Language:English
Published: EUROPEAN RESPIRATORY SOC JOURNALS LTD 2008
Subjects:
Science & Technology
Life Sciences & Biomedicine
Respiratory System
atopic asthma
atopy
housekeeping genes
human bronchial epithelial cells
real-time quantitative PCR
RT-PCR
BETA-ACTIN
EXPRESSION
AIRWAY
NORMALIZATION
ASTHMA
CYCLOPHILIN
CYTOKINE
INJURY
Online Access:http://hdl.handle.net/20.500.11937/76826

Internet

http://hdl.handle.net/20.500.11937/76826

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