Upper Airway Cell Transcriptomics Identify a Major New Immunological Phenotype with Strong Clinical Correlates in Young Children with Acute Wheezing.

Asthma exacerbations are triggered by rhinovirus infections. We employed a systems biology approach to delineate upper-airway gene network patterns underlying asthma exacerbation phenotypes in children. Cluster analysis unveiled distinct IRF7hi versus IRF7lo molecular phenotypes, the former exhibiti...

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Main Authors: Khoo, S., Read, J., Franks, K., Zhang, Guicheng, Bizzintino, J., Coleman, L., McCrae, C., Öberg, L., Troy, N., Prastanti, F., Everard, J., Oo, S., Borland, M., Maciewicz, R., Le Souëf, P., Laing, I., Bosco, A.
Format: Journal Article
Published: 2019
Online Access:http://hdl.handle.net/20.500.11937/74236
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author Khoo, S.
Read, J.
Franks, K.
Zhang, Guicheng
Bizzintino, J.
Coleman, L.
McCrae, C.
Öberg, L.
Troy, N.
Prastanti, F.
Everard, J.
Oo, S.
Borland, M.
Maciewicz, R.
Le Souëf, P.
Laing, I.
Bosco, A.
author_facet Khoo, S.
Read, J.
Franks, K.
Zhang, Guicheng
Bizzintino, J.
Coleman, L.
McCrae, C.
Öberg, L.
Troy, N.
Prastanti, F.
Everard, J.
Oo, S.
Borland, M.
Maciewicz, R.
Le Souëf, P.
Laing, I.
Bosco, A.
author_sort Khoo, S.
building Curtin Institutional Repository
collection Online Access
description Asthma exacerbations are triggered by rhinovirus infections. We employed a systems biology approach to delineate upper-airway gene network patterns underlying asthma exacerbation phenotypes in children. Cluster analysis unveiled distinct IRF7hi versus IRF7lo molecular phenotypes, the former exhibiting robust upregulation of Th1/type I IFN responses and the latter an alternative signature marked by upregulation of cytokine and growth factor signaling and downregulation of IFN-?. The two phenotypes also produced distinct clinical phenotypes. For IRF7lo children, symptom duration prior to hospital presentation was more than twice as long from initial symptoms (p = 0.011) and nearly three times as long for cough (p < 0.001), the odds ratio of admission to hospital was increased more than 4-fold (p = 0.018), and time to recurrence was shorter (p = 0.015). In summary, our findings demonstrate that asthma exacerbations in children can be divided into IRF7hi versus IRF7lo phenotypes with associated differences in clinical phenotypes.
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institution Curtin University Malaysia
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publishDate 2019
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spelling curtin-20.500.11937-742362019-02-19T04:25:51Z Upper Airway Cell Transcriptomics Identify a Major New Immunological Phenotype with Strong Clinical Correlates in Young Children with Acute Wheezing. Khoo, S. Read, J. Franks, K. Zhang, Guicheng Bizzintino, J. Coleman, L. McCrae, C. Öberg, L. Troy, N. Prastanti, F. Everard, J. Oo, S. Borland, M. Maciewicz, R. Le Souëf, P. Laing, I. Bosco, A. Asthma exacerbations are triggered by rhinovirus infections. We employed a systems biology approach to delineate upper-airway gene network patterns underlying asthma exacerbation phenotypes in children. Cluster analysis unveiled distinct IRF7hi versus IRF7lo molecular phenotypes, the former exhibiting robust upregulation of Th1/type I IFN responses and the latter an alternative signature marked by upregulation of cytokine and growth factor signaling and downregulation of IFN-?. The two phenotypes also produced distinct clinical phenotypes. For IRF7lo children, symptom duration prior to hospital presentation was more than twice as long from initial symptoms (p = 0.011) and nearly three times as long for cough (p < 0.001), the odds ratio of admission to hospital was increased more than 4-fold (p = 0.018), and time to recurrence was shorter (p = 0.015). In summary, our findings demonstrate that asthma exacerbations in children can be divided into IRF7hi versus IRF7lo phenotypes with associated differences in clinical phenotypes. 2019 Journal Article http://hdl.handle.net/20.500.11937/74236 10.4049/jimmunol.1800178 restricted
spellingShingle Khoo, S.
Read, J.
Franks, K.
Zhang, Guicheng
Bizzintino, J.
Coleman, L.
McCrae, C.
Öberg, L.
Troy, N.
Prastanti, F.
Everard, J.
Oo, S.
Borland, M.
Maciewicz, R.
Le Souëf, P.
Laing, I.
Bosco, A.
Upper Airway Cell Transcriptomics Identify a Major New Immunological Phenotype with Strong Clinical Correlates in Young Children with Acute Wheezing.
title Upper Airway Cell Transcriptomics Identify a Major New Immunological Phenotype with Strong Clinical Correlates in Young Children with Acute Wheezing.
title_full Upper Airway Cell Transcriptomics Identify a Major New Immunological Phenotype with Strong Clinical Correlates in Young Children with Acute Wheezing.
title_fullStr Upper Airway Cell Transcriptomics Identify a Major New Immunological Phenotype with Strong Clinical Correlates in Young Children with Acute Wheezing.
title_full_unstemmed Upper Airway Cell Transcriptomics Identify a Major New Immunological Phenotype with Strong Clinical Correlates in Young Children with Acute Wheezing.
title_short Upper Airway Cell Transcriptomics Identify a Major New Immunological Phenotype with Strong Clinical Correlates in Young Children with Acute Wheezing.
title_sort upper airway cell transcriptomics identify a major new immunological phenotype with strong clinical correlates in young children with acute wheezing.
url http://hdl.handle.net/20.500.11937/74236