Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes

QR Code

Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes

In type 2 diabetes, prolonged dysregulation of signalling and ß-cell metabolic control leads to ß-cell dysfunction, and is increasingly associated with abnormal metabolic states which disrupt normal cellular physiology. Utilization of appropriate ß-cell models enables a systematic approach to unders...

Full description

Bibliographic Details
Main Authors:	Rowlands, J., Walz, N., Rowles, J., Keane, Kevin, Carlessi, Rodrigo, Newsholme, P.
Format:	Journal Article
Published:	Humana Press 2019
Online Access:	http://hdl.handle.net/20.500.11937/74054

Similar Items

Insulin and IGF-1 receptor autocrine loops are not required for Exendin-4 induced changes to pancreatic ß-cell bioenergetic parameters and metabolism in BRIN-BD11 cells
by: Rowlands, Jordan, et al.
Published: (2018)

Pigment epithelium-derived factor (PEDF) regulates metabolism and insulin secretion from a clonal rat pancreatic beta cell line BRIN-BD11 and mouse islets
by: Chen, Y., et al.
Published: (2016)

Lupin seed hydrolysate promotes G-protein-coupled receptor, intracellular Ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from BRIN-BD11 pancreatic beta cells
by: Tapadia, M., et al.
Published: (2018)

Toll-like receptor agonist induced changes in clonal rat BRIN-BD11 ß-cell insulin secretion and signal transduction
by: Kiely, A., et al.
Published: (2009)

Overexpression of the malate-aspartate NADH shuttle member Aralar1 in the clonal ß-cell line BRIN-BD11 enhances amino-acid-stimulated insulin secretion and cell metabolism
by: Bender, K., et al.
Published: (2009)

The growth culture of BRIN-BD11 producing insulin in different type of microcarriers
by: Mel, Maizirwan, et al.
Published: (2011)

Cytotoxicity and cytoprotective effects of citrus flavonoids on insulin-secreting cells BRIN-BD11: beneficial synergic effects.
by: Felipe, E., et al.
Published: (2013)

Comparing BRIN-BD11 culture producing insulin using different type of microcarriers
by: Mel, Maizirwan, et al.
Published: (2010)

Effects of oil palm (Elaeis guineensis) fruit extracts on insulin secretion from BRIN BD11 cells
by: Sharif, Faez, et al.
Published: (2015)

Nutrient regulation of ß-cell function: what do islet cell/animal studies tell us?
by: Carlessi, Rodrigo, et al.
Published: (2017)

Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion
by: Abdul Karim, Mohamed Ismail
Published: (2009)

Molecular Events Linking Oxidative Stress and Inflammation to Insulin Resistance and ß-Cell Dysfunction
by: Keane, Kevin, et al.
Published: (2015)

Glutamine deprivation induces metabolic adaptations associated with beta cell dysfunction and exacerbate lipotoxicity
by: Carlessi, Rodrigo, et al.
Published: (2018)

The Impact of Inflammation on Pancreatic ß-Cell Metabolism, Function and Failure in T1DM and T2DM: Commonalities and Differences
by: Newsholme, Philip, et al.
Published: (2013)

Metabolic Regulation of Insulin Secretion
by: Keane, Kevin, et al.
Published: (2014)

The bioenergetics of inflammation: insights into obesity and type 2 diabetes
by: Keane, Kevin, et al.
Published: (2017)

GLP-1 receptor signalling promotes β-cell glucose metabolism via mTOR-dependent HIF-1α activation
by: Carlessi, Rodrigo, et al.
Published: (2017)

Molecular mechanisms of ROS production and oxidative stress in diabetes
by: Newsholme, Philip, et al.
Published: (2016)

Preclinical pharmacokinetics, pharmacodynamics, and toxicity of novel small-molecule GPR119 agonists to treat type-2 diabetes and obesity
by: Patil, Mohan, et al.
Published: (2024)

Saturated and unsaturated (including arachidonic acid) non-esterified fatty acid modulation of insulin secretion from pancreatic ß-cells
by: Keane, D., et al.
Published: (2008)

Alanyl-glutamine improves pancreatic ß-cell function following ex vivo inflammatory challenge
by: Cruzat, Vinicius, et al.
Published: (2015)

Metabolomic analyses reveal profound differences in glycolytic and tricarboxylic acid cycle metabolism in glucose-responsive and -unresponsive clonal ß-cell lines
by: Spegel, P., et al.
Published: (2011)

Glutamine: Metabolism and immune function, supplementation and clinical translation
by: Cruzat, Vinicius, et al.
Published: (2018)

The utility of anatomy assessment in preclinical undergraduate medical curriculum: a scoping review protocol
by: Alias, Aspalilah, et al.
Published: (2024)

UMPSA strengthens research ties with Indonesia’s National Research and Innovation Agency (BRIN)
by: Mirta, Widia
Published: (2025)

Preclinical evidence of Malay traditional herbal supplement for diabetes
by: Abdul Majid, Fadzilah Adibah, et al.
Published: (2016)

Arachidonic acid actions on functional integrity and attenuation of the negative effects of palmitic acid in a clonal pancreatic ß-cell line
by: Keane, D., et al.
Published: (2011)

Prolonged L-alanine exposure induces changes in metabolism, Ca2+ handling and desensitization of insulin secretion in clonal pancreatic ß-cells
by: McClenaghan, N., et al.
Published: (2009)

SS Efficient execution vital for policies' success, 11 May 2013
by: New Sarawak, Tribune
Published: (2013)

Intrinsic distortion of a fully differential BD-modulated Class-D amplifier with analog feedback
by: Cox, Stephen M., et al.
Published: (2013)

Higher ß-HCG concentrations and higher birthweights ensue from single vitrified embryo transfers
by: Keane, Kevin, et al.
Published: (2015)

β-cell metabolism, insulin production and secretion: Metabolic failure resulting in diabetes
by: Chen, Y., et al.
Published: (2016)

Prevailing vitamin D status influences mitochondrial and glycolytic bioenergetics in peripheral blood mononuclear cells obtained from adults
by: Calton, Emily, et al.
Published: (2016)

Preclinical safety evaluation of WJMSCs and their secretome
by: Premasangery Kathivaloo,, et al.
Published: (2025)

Winter to summer change in vitamin D status reduces systemic inflammation and bioenergetic activity of human peripheral blood mononuclear cells
by: Calton, E., et al.
Published: (2017)

Wnt/ß-Catenin Signalling during Liver Metabolism, Chronic Liver Disease and Hepatocarcinogenesis
by: Shirolkar, Gayatri, et al.
Published: (2018)

Development of cordycepin formulations for preclinical and clinical studies
by: Lee, Jong Bong, et al.
Published: (2017)

Evaluation of the BD GeneOhm MRSA ACP Assay and the Cepheid GeneXpert MRSA Assay to detect genetically diverse CA-MRSA.
by: Coombs, Geoffrey, et al.
Published: (2013)

Elevated levels of extracellular heat-shock protein 72 (eHSP72) are positively correlated with insulin resistance in vivo and cause pancreatic ß-cell dysfunction and death in vitro.
by: Krause, M., et al.
Published: (2013)

Nutrient regulation of insulin secretion and ß-cell functional integrity
by: Newsholme, Philip, et al.
Published: (2010)