Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes
In type 2 diabetes, prolonged dysregulation of signalling and ß-cell metabolic control leads to ß-cell dysfunction, and is increasingly associated with abnormal metabolic states which disrupt normal cellular physiology. Utilization of appropriate ß-cell models enables a systematic approach to unders...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Published: |
Humana Press
2019
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| Online Access: | http://hdl.handle.net/20.500.11937/74054 |
| _version_ | 1848763167690194944 |
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| author | Rowlands, J. Walz, N. Rowles, J. Keane, Kevin Carlessi, Rodrigo Newsholme, P. |
| author_facet | Rowlands, J. Walz, N. Rowles, J. Keane, Kevin Carlessi, Rodrigo Newsholme, P. |
| author_sort | Rowlands, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In type 2 diabetes, prolonged dysregulation of signalling and ß-cell metabolic control leads to ß-cell dysfunction, and is increasingly associated with abnormal metabolic states which disrupt normal cellular physiology. Utilization of appropriate ß-cell models enables a systematic approach to understand the impact of perturbations to the biological system. The BRIN-BD11 ß-cell line is a useful, pre-clinical cell model for ß-cell dysfunction associated with type 2 diabetes, among other metabolic disorders. The present chapter describes detection and analysis of ß-cell dysfunction with respect to changes in bioenergetics and metabolism, generation of intracellular reactive oxygen species, and acute and chronic insulin secretion in the BRIN-BD11 cell line. |
| first_indexed | 2025-11-14T10:59:10Z |
| format | Journal Article |
| id | curtin-20.500.11937-74054 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:59:10Z |
| publishDate | 2019 |
| publisher | Humana Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-740542019-07-31T05:37:55Z Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes Rowlands, J. Walz, N. Rowles, J. Keane, Kevin Carlessi, Rodrigo Newsholme, P. In type 2 diabetes, prolonged dysregulation of signalling and ß-cell metabolic control leads to ß-cell dysfunction, and is increasingly associated with abnormal metabolic states which disrupt normal cellular physiology. Utilization of appropriate ß-cell models enables a systematic approach to understand the impact of perturbations to the biological system. The BRIN-BD11 ß-cell line is a useful, pre-clinical cell model for ß-cell dysfunction associated with type 2 diabetes, among other metabolic disorders. The present chapter describes detection and analysis of ß-cell dysfunction with respect to changes in bioenergetics and metabolism, generation of intracellular reactive oxygen species, and acute and chronic insulin secretion in the BRIN-BD11 cell line. 2019 Journal Article http://hdl.handle.net/20.500.11937/74054 10.1007/978-1-4939-8994-2_32 Humana Press restricted |
| spellingShingle | Rowlands, J. Walz, N. Rowles, J. Keane, Kevin Carlessi, Rodrigo Newsholme, P. Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes |
| title | Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes |
| title_full | Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes |
| title_fullStr | Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes |
| title_full_unstemmed | Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes |
| title_short | Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line: A Preclinical Model for Type 2 Diabetes |
| title_sort | method protocols for metabolic and functional analysis of the brin-bd11 ß-cell line: a preclinical model for type 2 diabetes |
| url | http://hdl.handle.net/20.500.11937/74054 |