Characterization of phenolic compounds and antioxidant activity in sorghum grains

Phenolic composition and relative antioxidant activity were investigated in six varieties of sorghum including pigmented and non-pigmented pericarp varieties. Whole grain sorghum extracts were subjected to acidified acetone extraction and were analysed using UHPLC with an attached online ABTS system...

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Main Authors: Rao, S., Santhakumar, A., Chinkwo, K., Wu, G., Johnson, Stuart, Blanchard, C.
Format: Journal Article
Published: Academic Press 2018
Online Access:http://hdl.handle.net/20.500.11937/73034
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author Rao, S.
Santhakumar, A.
Chinkwo, K.
Wu, G.
Johnson, Stuart
Blanchard, C.
author_facet Rao, S.
Santhakumar, A.
Chinkwo, K.
Wu, G.
Johnson, Stuart
Blanchard, C.
author_sort Rao, S.
building Curtin Institutional Repository
collection Online Access
description Phenolic composition and relative antioxidant activity were investigated in six varieties of sorghum including pigmented and non-pigmented pericarp varieties. Whole grain sorghum extracts were subjected to acidified acetone extraction and were analysed using UHPLC with an attached online ABTS system and Q-TOF LC/MS. Results showed that the black pericarp variety Shawaya short black 1 and the brown pericarp variety IS11316 had the highest total phenolic content (TPC) and total proanthocyanidin content (TPAC), consequently resulting in overall high antioxidant activity. The phenolic compounds that had the highest antioxidant activity in Shawaya and IS11316 were catechin, 1-O-cafferolglycerol-O-glucoside, taxifolin and pentahydroxyflavanone-(3->4)-catechin-7-O-glucoside. Furthermore, comparison of the phenolic profiles has shown that the varieties with higher antioxidant activity generally had higher quantities and more diverse range of polyphenols. Thus, this study indicates that there is no independent compound in sorghum that results in its high antioxidant activity but rather it is the cumulative effect of various phenolic compounds.
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spelling curtin-20.500.11937-730342019-02-12T03:01:02Z Characterization of phenolic compounds and antioxidant activity in sorghum grains Rao, S. Santhakumar, A. Chinkwo, K. Wu, G. Johnson, Stuart Blanchard, C. Phenolic composition and relative antioxidant activity were investigated in six varieties of sorghum including pigmented and non-pigmented pericarp varieties. Whole grain sorghum extracts were subjected to acidified acetone extraction and were analysed using UHPLC with an attached online ABTS system and Q-TOF LC/MS. Results showed that the black pericarp variety Shawaya short black 1 and the brown pericarp variety IS11316 had the highest total phenolic content (TPC) and total proanthocyanidin content (TPAC), consequently resulting in overall high antioxidant activity. The phenolic compounds that had the highest antioxidant activity in Shawaya and IS11316 were catechin, 1-O-cafferolglycerol-O-glucoside, taxifolin and pentahydroxyflavanone-(3->4)-catechin-7-O-glucoside. Furthermore, comparison of the phenolic profiles has shown that the varieties with higher antioxidant activity generally had higher quantities and more diverse range of polyphenols. Thus, this study indicates that there is no independent compound in sorghum that results in its high antioxidant activity but rather it is the cumulative effect of various phenolic compounds. 2018 Journal Article http://hdl.handle.net/20.500.11937/73034 10.1016/j.jcs.2018.07.013 Academic Press restricted
spellingShingle Rao, S.
Santhakumar, A.
Chinkwo, K.
Wu, G.
Johnson, Stuart
Blanchard, C.
Characterization of phenolic compounds and antioxidant activity in sorghum grains
title Characterization of phenolic compounds and antioxidant activity in sorghum grains
title_full Characterization of phenolic compounds and antioxidant activity in sorghum grains
title_fullStr Characterization of phenolic compounds and antioxidant activity in sorghum grains
title_full_unstemmed Characterization of phenolic compounds and antioxidant activity in sorghum grains
title_short Characterization of phenolic compounds and antioxidant activity in sorghum grains
title_sort characterization of phenolic compounds and antioxidant activity in sorghum grains
url http://hdl.handle.net/20.500.11937/73034