HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay
Inheritance of HLA-B*5701 is a strong predictor of a hypersensitivity reaction to the anti-HIV drug abacavir. The identification of susceptible individuals prior to the institution of abacavir therapy is therefore of clinical importance and has generated demand for a simple and rapid diagnostic test...
| Main Authors: | , , , |
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| Format: | Journal Article |
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Blackwell Munksgaard
2007
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| Online Access: | http://hdl.handle.net/20.500.11937/5945 |
| _version_ | 1848744937859842048 |
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| author | Hammond, E. Cyril Nolan, D. Mallal, S. |
| author_facet | Hammond, E. Cyril Nolan, D. Mallal, S. |
| author_sort | Hammond, E. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Inheritance of HLA-B*5701 is a strong predictor of a hypersensitivity reaction to the anti-HIV drug abacavir. The identification of susceptible individuals prior to the institution of abacavir therapy is therefore of clinical importance and has generated demand for a simple and rapid diagnostic test for carriage of HLA-B*5701. In this study, we describe the development of such a method based on allele-specific polymerase chain reaction (AS-PCR) and melting curve analysis. Ninety-six patient samples including 36 HLA-B*5701-positive samples and 60 HLA-B*5701-negative samples were analysed. Compared with sequence-based typing, this method had 100% sensitivity and specificity for the HLA-B*5701 allele. In conclusion, the AS-PCR/melting curve approach minimises post-polymerase chain reaction handling processing and provides an attractive alternative to currently described AS-PCR methods. |
| first_indexed | 2025-11-14T06:09:24Z |
| format | Journal Article |
| id | curtin-20.500.11937-5945 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:09:24Z |
| publishDate | 2007 |
| publisher | Blackwell Munksgaard |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-59452017-09-13T16:06:09Z HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay Hammond, E. Cyril Nolan, D. Mallal, S. Inheritance of HLA-B*5701 is a strong predictor of a hypersensitivity reaction to the anti-HIV drug abacavir. The identification of susceptible individuals prior to the institution of abacavir therapy is therefore of clinical importance and has generated demand for a simple and rapid diagnostic test for carriage of HLA-B*5701. In this study, we describe the development of such a method based on allele-specific polymerase chain reaction (AS-PCR) and melting curve analysis. Ninety-six patient samples including 36 HLA-B*5701-positive samples and 60 HLA-B*5701-negative samples were analysed. Compared with sequence-based typing, this method had 100% sensitivity and specificity for the HLA-B*5701 allele. In conclusion, the AS-PCR/melting curve approach minimises post-polymerase chain reaction handling processing and provides an attractive alternative to currently described AS-PCR methods. 2007 Journal Article http://hdl.handle.net/20.500.11937/5945 10.1111/j.1399-0039.2007.00840.x Blackwell Munksgaard restricted |
| spellingShingle | Hammond, E. Cyril Nolan, D. Mallal, S. HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay |
| title | HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay |
| title_full | HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay |
| title_fullStr | HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay |
| title_full_unstemmed | HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay |
| title_short | HLA-B*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay |
| title_sort | hla-b*5701 typing: evaluation of an allele-specific polymerase chain reaction melting assay |
| url | http://hdl.handle.net/20.500.11937/5945 |