Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels
The spatial presentation of mechanical information is a key parameter for cell behavior. We have developed a method of polymerization control in which the differential diffusion distance of unreacted cross-linker and monomer into a prepolymerized hydrogel sink results in a tunable stiffness gradient...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
National Academy of Sciences
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/56114 |
| _version_ | 1848759790000406528 |
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| author | Hadden, W. Young, J. Holle, A. McFetridge, M. Kim, Du Yong Wijesinghe, P. Taylor-Weiner, H. Wen, J. Lee, A. Bieback, K. Vo, Ba-Ngu Sampson, D. Kennedy, B. Spatz, J. Engler, A. Cho, Y. |
| author_facet | Hadden, W. Young, J. Holle, A. McFetridge, M. Kim, Du Yong Wijesinghe, P. Taylor-Weiner, H. Wen, J. Lee, A. Bieback, K. Vo, Ba-Ngu Sampson, D. Kennedy, B. Spatz, J. Engler, A. Cho, Y. |
| author_sort | Hadden, W. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The spatial presentation of mechanical information is a key parameter for cell behavior. We have developed a method of polymerization control in which the differential diffusion distance of unreacted cross-linker and monomer into a prepolymerized hydrogel sink results in a tunable stiffness gradient at the cell-matrix interface. This simple, low-cost, robust method was used to produce polyacrylamide hydrogels with stiffness gradients of 0.5, 1.7, 2.9, 4.5, 6.8, and 8.2 kPa/mm, spanning the in vivo physiological and pathological mechanical landscape. Importantly, three of these gradients were found to be nondurotactic for human adipose-derived stem cells (hASCs), allowing the presentation of a continuous range of stiffnesses in a single well without the confounding effect of differential cell migration. Using these nondurotactic gradient gels, stiffness-dependent hASC morphology, migration, and differentiation were studied. Finally, the mechanosensitive proteins YAP, Lamin A/C, Lamin B, MRTF-A, and MRTF-B were analyzed on these gradients, providing higher-resolution data on stiffness-dependent expression and localization. |
| first_indexed | 2025-11-14T10:05:28Z |
| format | Journal Article |
| id | curtin-20.500.11937-56114 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:05:28Z |
| publishDate | 2017 |
| publisher | National Academy of Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-561142023-02-22T06:24:22Z Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels Hadden, W. Young, J. Holle, A. McFetridge, M. Kim, Du Yong Wijesinghe, P. Taylor-Weiner, H. Wen, J. Lee, A. Bieback, K. Vo, Ba-Ngu Sampson, D. Kennedy, B. Spatz, J. Engler, A. Cho, Y. The spatial presentation of mechanical information is a key parameter for cell behavior. We have developed a method of polymerization control in which the differential diffusion distance of unreacted cross-linker and monomer into a prepolymerized hydrogel sink results in a tunable stiffness gradient at the cell-matrix interface. This simple, low-cost, robust method was used to produce polyacrylamide hydrogels with stiffness gradients of 0.5, 1.7, 2.9, 4.5, 6.8, and 8.2 kPa/mm, spanning the in vivo physiological and pathological mechanical landscape. Importantly, three of these gradients were found to be nondurotactic for human adipose-derived stem cells (hASCs), allowing the presentation of a continuous range of stiffnesses in a single well without the confounding effect of differential cell migration. Using these nondurotactic gradient gels, stiffness-dependent hASC morphology, migration, and differentiation were studied. Finally, the mechanosensitive proteins YAP, Lamin A/C, Lamin B, MRTF-A, and MRTF-B were analyzed on these gradients, providing higher-resolution data on stiffness-dependent expression and localization. 2017 Journal Article http://hdl.handle.net/20.500.11937/56114 10.1073/pnas.1618239114 National Academy of Sciences unknown |
| spellingShingle | Hadden, W. Young, J. Holle, A. McFetridge, M. Kim, Du Yong Wijesinghe, P. Taylor-Weiner, H. Wen, J. Lee, A. Bieback, K. Vo, Ba-Ngu Sampson, D. Kennedy, B. Spatz, J. Engler, A. Cho, Y. Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels |
| title | Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels |
| title_full | Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels |
| title_fullStr | Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels |
| title_full_unstemmed | Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels |
| title_short | Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels |
| title_sort | stem cell migration and mechanotransduction on linear stiffness gradient hydrogels |
| url | http://hdl.handle.net/20.500.11937/56114 |