Construction of an integration vector carrying hygromycin B resistance gene and its genetic transformation in Rhizopus oryzae

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Construction of an integration vector carrying hygromycin B resistance gene and its genetic transformation in Rhizopus oryzae

To construct a system of genetic transformation suitable for Rhizopus oryzae, we constructed a single-exchange vector pBS-hygro carrying hygromycin B resistance gene (hph) as its selective marker using gene splicing by overlap extension PCR (SOE PCR) technique. We introduced this recombinant vector...

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Bibliographic Details
Main Authors:	Zhang, M., Jiang, S., Zheng, J., Zheng, Z., Li, Xingjiang, Pan, L., Luo, S.
Format:	Journal Article
Published:	Chinese Academy of Sciences 2015
Online Access:	http://hdl.handle.net/20.500.11937/4904

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