Construction of an integration vector carrying hygromycin B resistance gene and its genetic transformation in Rhizopus oryzae
To construct a system of genetic transformation suitable for Rhizopus oryzae, we constructed a single-exchange vector pBS-hygro carrying hygromycin B resistance gene (hph) as its selective marker using gene splicing by overlap extension PCR (SOE PCR) technique. We introduced this recombinant vector...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Published: |
Chinese Academy of Sciences
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/4904 |