High-throughput ß-galactosidase and ß-glucuronidase Assays Using Fluorogenic Substrates

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High-throughput ß-galactosidase and ß-glucuronidase Assays Using Fluorogenic Substrates

β-galactosidase and β-glucuronidase enzymes are commonly used as reporters for gene expression from gene promoter-lacZ or uidA fusions (respectively). The protocol described here is a high-throughput alternative to the commonly used Miller assay (Miller, 1972) that utilises a fluorogenic substrate (...

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Bibliographic Details
Main Author:	Ramsay, Joshua
Format:	Journal Article
Published:	2013
Online Access:	http://hdl.handle.net/20.500.11937/46320

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