High-throughput ß-galactosidase and ß-glucuronidase Assays Using Fluorogenic Substrates
β-galactosidase and β-glucuronidase enzymes are commonly used as reporters for gene expression from gene promoter-lacZ or uidA fusions (respectively). The protocol described here is a high-throughput alternative to the commonly used Miller assay (Miller, 1972) that utilises a fluorogenic substrate (...
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| Format: | Journal Article |
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2013
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| Online Access: | http://hdl.handle.net/20.500.11937/46320 |