Receptor activator of NF-κB ligand promotes proliferation of a putative mammary stem cell unique to the lactating epithelium
In mice, CD49fhi mammary stem cells (MaSCs) asymmetrically divide to generate CD49f+ committed progenitor cells that differentiate into CD49f− phenotypes of the milk-secreting tissue at the onset of pregnancy. We show CD49f+ primary mammary epithelial cells (PMECs) isolated from lactating tissue uni...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Published: |
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/45110 |
| _version_ | 1848757191689895936 |
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| author | Thomas, E. Lee-Pullen, T. Rigby, P. Hartmann, P. Xu, J. Zeps, Nikolajs |
| author_facet | Thomas, E. Lee-Pullen, T. Rigby, P. Hartmann, P. Xu, J. Zeps, Nikolajs |
| author_sort | Thomas, E. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In mice, CD49fhi mammary stem cells (MaSCs) asymmetrically divide to generate CD49f+ committed progenitor cells that differentiate into CD49f− phenotypes of the milk-secreting tissue at the onset of pregnancy. We show CD49f+ primary mammary epithelial cells (PMECs) isolated from lactating tissue uniquely respond to pregnancy-associated hormones (PAH) compared with CD49f+ cells from nonlactating tissue. Differentiation of CD49f+ PMEC in extracellular matrix produces CD49f− luminal cells to form differentiated alveoli. The PAH prolactin and placental lactogen specifically stimulate division of CD49f− luminal cells, while receptor activator of nuclear factor (NF)-κB ligand (RANKL) specifically stimulates division of basal CD49f+ cells. In nondifferentiating conditions, we observed a greater proportion of multipotent self-renewing cells, and RANKL treatment activated the RANK pathway in these cultures. Furthermore, we observed the deposition of calcium nodules in a proportion of these cells. These data imply that a MaSC unique to the lactating breast exists in humans, which generates progeny with discrete lineages and distinct response to PAH. |
| first_indexed | 2025-11-14T09:24:10Z |
| format | Journal Article |
| id | curtin-20.500.11937-45110 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:24:10Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-451102017-09-13T14:18:22Z Receptor activator of NF-κB ligand promotes proliferation of a putative mammary stem cell unique to the lactating epithelium Thomas, E. Lee-Pullen, T. Rigby, P. Hartmann, P. Xu, J. Zeps, Nikolajs In mice, CD49fhi mammary stem cells (MaSCs) asymmetrically divide to generate CD49f+ committed progenitor cells that differentiate into CD49f− phenotypes of the milk-secreting tissue at the onset of pregnancy. We show CD49f+ primary mammary epithelial cells (PMECs) isolated from lactating tissue uniquely respond to pregnancy-associated hormones (PAH) compared with CD49f+ cells from nonlactating tissue. Differentiation of CD49f+ PMEC in extracellular matrix produces CD49f− luminal cells to form differentiated alveoli. The PAH prolactin and placental lactogen specifically stimulate division of CD49f− luminal cells, while receptor activator of nuclear factor (NF)-κB ligand (RANKL) specifically stimulates division of basal CD49f+ cells. In nondifferentiating conditions, we observed a greater proportion of multipotent self-renewing cells, and RANKL treatment activated the RANK pathway in these cultures. Furthermore, we observed the deposition of calcium nodules in a proportion of these cells. These data imply that a MaSC unique to the lactating breast exists in humans, which generates progeny with discrete lineages and distinct response to PAH. 2012 Journal Article http://hdl.handle.net/20.500.11937/45110 10.1002/stem.1092 unknown |
| spellingShingle | Thomas, E. Lee-Pullen, T. Rigby, P. Hartmann, P. Xu, J. Zeps, Nikolajs Receptor activator of NF-κB ligand promotes proliferation of a putative mammary stem cell unique to the lactating epithelium |
| title | Receptor activator of NF-κB ligand promotes proliferation of a putative mammary stem cell unique to the lactating epithelium |
| title_full | Receptor activator of NF-κB ligand promotes proliferation of a putative mammary stem cell unique to the lactating epithelium |
| title_fullStr | Receptor activator of NF-κB ligand promotes proliferation of a putative mammary stem cell unique to the lactating epithelium |
| title_full_unstemmed | Receptor activator of NF-κB ligand promotes proliferation of a putative mammary stem cell unique to the lactating epithelium |
| title_short | Receptor activator of NF-κB ligand promotes proliferation of a putative mammary stem cell unique to the lactating epithelium |
| title_sort | receptor activator of nf-κb ligand promotes proliferation of a putative mammary stem cell unique to the lactating epithelium |
| url | http://hdl.handle.net/20.500.11937/45110 |