Synthesis and Antileukemic Activity of New Fluorinated 5-Aza-9-deazapurines

Novel fluorinated 4-benzylamino-7-phenylpyrazolo[1,5-a]- [1,3,5]triazin-2-amines were prepared using an efficient and practical approach. The chemoselectivity of condensation of pyrazolylguanidine and trichloroacetonitrile was found to be solvent-dependent and, when conducted in toluene, this reacti...

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Main Authors: Lim, F., Kow, K., Yeo, E., Chow, S., Dolzhenko, Anton
Format: Journal Article
Published: The Japan Institute of Heterocyclic Chemistry 2016
Online Access:http://hdl.handle.net/20.500.11937/44647
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author Lim, F.
Kow, K.
Yeo, E.
Chow, S.
Dolzhenko, Anton
author_facet Lim, F.
Kow, K.
Yeo, E.
Chow, S.
Dolzhenko, Anton
author_sort Lim, F.
building Curtin Institutional Repository
collection Online Access
description Novel fluorinated 4-benzylamino-7-phenylpyrazolo[1,5-a]- [1,3,5]triazin-2-amines were prepared using an efficient and practical approach. The chemoselectivity of condensation of pyrazolylguanidine and trichloroacetonitrile was found to be solvent-dependent and, when conducted in toluene, this reaction afforded 7-phenyl-4-trichloromethylpyrazolo[1,5-a]- [1,3,5]triazin-2-amine as the main product. This key intermediate underwent nucleophilic replacement of the trichloromethyl group with fluorinated benzylamines providing a series of the target compounds. Antiproliferative activity of the prepared compounds against Jurkat T cells was explored using MTS assay. Morphological changes observed in cells treated by the most potent compounds of this series, suggested that these compounds induced apoptosis in cells.
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publisher The Japan Institute of Heterocyclic Chemistry
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spelling curtin-20.500.11937-446472017-09-13T14:10:55Z Synthesis and Antileukemic Activity of New Fluorinated 5-Aza-9-deazapurines Lim, F. Kow, K. Yeo, E. Chow, S. Dolzhenko, Anton Novel fluorinated 4-benzylamino-7-phenylpyrazolo[1,5-a]- [1,3,5]triazin-2-amines were prepared using an efficient and practical approach. The chemoselectivity of condensation of pyrazolylguanidine and trichloroacetonitrile was found to be solvent-dependent and, when conducted in toluene, this reaction afforded 7-phenyl-4-trichloromethylpyrazolo[1,5-a]- [1,3,5]triazin-2-amine as the main product. This key intermediate underwent nucleophilic replacement of the trichloromethyl group with fluorinated benzylamines providing a series of the target compounds. Antiproliferative activity of the prepared compounds against Jurkat T cells was explored using MTS assay. Morphological changes observed in cells treated by the most potent compounds of this series, suggested that these compounds induced apoptosis in cells. 2016 Journal Article http://hdl.handle.net/20.500.11937/44647 10.3987/COM-16-13464 The Japan Institute of Heterocyclic Chemistry restricted
spellingShingle Lim, F.
Kow, K.
Yeo, E.
Chow, S.
Dolzhenko, Anton
Synthesis and Antileukemic Activity of New Fluorinated 5-Aza-9-deazapurines
title Synthesis and Antileukemic Activity of New Fluorinated 5-Aza-9-deazapurines
title_full Synthesis and Antileukemic Activity of New Fluorinated 5-Aza-9-deazapurines
title_fullStr Synthesis and Antileukemic Activity of New Fluorinated 5-Aza-9-deazapurines
title_full_unstemmed Synthesis and Antileukemic Activity of New Fluorinated 5-Aza-9-deazapurines
title_short Synthesis and Antileukemic Activity of New Fluorinated 5-Aza-9-deazapurines
title_sort synthesis and antileukemic activity of new fluorinated 5-aza-9-deazapurines
url http://hdl.handle.net/20.500.11937/44647