A quantitative PCR approach to determine gene copy number
Here, we report on the use of quantitative PCR (qPCR) to determine gene copy number in filamentous fungi. Using the sequenced dothideomycete Stagonospora nodorum, qPCR was used to unequivocally confirm the presence of single, two and three copy regions as predicted by in silico PCR. Further validati...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
2008
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| Online Access: | http://hdl.handle.net/20.500.11937/44274 |
| _version_ | 1848756953317113856 |
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| author | Solomon, P. Ipcho, S. Hane, J. Tan, Kar-Chun Oliver, Richard |
| author_facet | Solomon, P. Ipcho, S. Hane, J. Tan, Kar-Chun Oliver, Richard |
| author_sort | Solomon, P. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Here, we report on the use of quantitative PCR (qPCR) to determine gene copy number in filamentous fungi. Using the sequenced dothideomycete Stagonospora nodorum, qPCR was used to unequivocally confirm the presence of single, two and three copy regions as predicted by in silico PCR. Further validation of the technique was demonstrated by verifying the copy numbers of introduced gene cassettes in previously characterised transformants of S. nodorum. Apart from increased sensitivity, this technique offers a high-throughput alternative to Southern blots for determining gene copy number, a significant factor when screening fungal mutants and transformants. |
| first_indexed | 2025-11-14T09:20:23Z |
| format | Journal Article |
| id | curtin-20.500.11937-44274 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:20:23Z |
| publishDate | 2008 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-442742017-01-30T15:13:09Z A quantitative PCR approach to determine gene copy number Solomon, P. Ipcho, S. Hane, J. Tan, Kar-Chun Oliver, Richard Here, we report on the use of quantitative PCR (qPCR) to determine gene copy number in filamentous fungi. Using the sequenced dothideomycete Stagonospora nodorum, qPCR was used to unequivocally confirm the presence of single, two and three copy regions as predicted by in silico PCR. Further validation of the technique was demonstrated by verifying the copy numbers of introduced gene cassettes in previously characterised transformants of S. nodorum. Apart from increased sensitivity, this technique offers a high-throughput alternative to Southern blots for determining gene copy number, a significant factor when screening fungal mutants and transformants. 2008 Journal Article http://hdl.handle.net/20.500.11937/44274 restricted |
| spellingShingle | Solomon, P. Ipcho, S. Hane, J. Tan, Kar-Chun Oliver, Richard A quantitative PCR approach to determine gene copy number |
| title | A quantitative PCR approach to determine gene copy number |
| title_full | A quantitative PCR approach to determine gene copy number |
| title_fullStr | A quantitative PCR approach to determine gene copy number |
| title_full_unstemmed | A quantitative PCR approach to determine gene copy number |
| title_short | A quantitative PCR approach to determine gene copy number |
| title_sort | quantitative pcr approach to determine gene copy number |
| url | http://hdl.handle.net/20.500.11937/44274 |