A quantitative PCR approach to determine gene copy number

Here, we report on the use of quantitative PCR (qPCR) to determine gene copy number in filamentous fungi. Using the sequenced dothideomycete Stagonospora nodorum, qPCR was used to unequivocally confirm the presence of single, two and three copy regions as predicted by in silico PCR. Further validati...

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Bibliographic Details
Main Authors: Solomon, P., Ipcho, S., Hane, J., Tan, Kar-Chun, Oliver, Richard
Format: Journal Article
Published: 2008
Online Access:http://hdl.handle.net/20.500.11937/44274