Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice
Apolipoprotein E (ApoE) is important in facilitating the transport of lipids (cholesterol, phospholipids, and sulfatides) and plays a fundamental role in normal lipid metabolism. High cholesterol levels increases the risk of developing Alzheimer’s disease. In this study, we investigated the effects...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
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Elsevier Inc.
2013
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/44156 |
| _version_ | 1848756916499513344 |
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| author | Lim, Wei Ling Lam, Sin Man Shui, Guanghou Mondal, Alinda Ong, Daniel Duan, Xinrui Creegan, Rhona martins, Ian Sharman, Matthew Taddei, Kevin Verdile, Giuseppe Wenk, Markus Martins, Ralph |
| author_facet | Lim, Wei Ling Lam, Sin Man Shui, Guanghou Mondal, Alinda Ong, Daniel Duan, Xinrui Creegan, Rhona martins, Ian Sharman, Matthew Taddei, Kevin Verdile, Giuseppe Wenk, Markus Martins, Ralph |
| author_sort | Lim, Wei Ling |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Apolipoprotein E (ApoE) is important in facilitating the transport of lipids (cholesterol, phospholipids, and sulfatides) and plays a fundamental role in normal lipid metabolism. High cholesterol levels increases the risk of developing Alzheimer’s disease. In this study, we investigated the effects of a high-fat high cholesterol (HFHC) diet on brain lipid profiles in 95 young and aged APOE ε3 and ε4 knock-in mice to determine whether diet leads to altered brain levels of a number of glycerophospholipids, sphingolipids, cholesterol precursors, cholesterol, cholesterol oxidation products, and cholesterol esters. The results in this study revealed significant changes in lipid levels. The HFHC-enriched diet influenced the levels of cholesterol esters. A sharp increase in cholesterol ester levels, particularly in the aged APOE ε4 diet-enriched group, might be suggestive of abnormal acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT) activity and/or levels. Age exerts appreciable effects on the brain lipidome, especially with regard to polar lipid species. |
| first_indexed | 2025-11-14T09:19:48Z |
| format | Journal Article |
| id | curtin-20.500.11937-44156 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:19:48Z |
| publishDate | 2013 |
| publisher | Elsevier Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-441562017-09-13T14:28:47Z Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice Lim, Wei Ling Lam, Sin Man Shui, Guanghou Mondal, Alinda Ong, Daniel Duan, Xinrui Creegan, Rhona martins, Ian Sharman, Matthew Taddei, Kevin Verdile, Giuseppe Wenk, Markus Martins, Ralph High cholesterol Mass spectrometry High fat Oxysterols APOE KI mice Glycerophospholipids Apolipoprotein E (ApoE) is important in facilitating the transport of lipids (cholesterol, phospholipids, and sulfatides) and plays a fundamental role in normal lipid metabolism. High cholesterol levels increases the risk of developing Alzheimer’s disease. In this study, we investigated the effects of a high-fat high cholesterol (HFHC) diet on brain lipid profiles in 95 young and aged APOE ε3 and ε4 knock-in mice to determine whether diet leads to altered brain levels of a number of glycerophospholipids, sphingolipids, cholesterol precursors, cholesterol, cholesterol oxidation products, and cholesterol esters. The results in this study revealed significant changes in lipid levels. The HFHC-enriched diet influenced the levels of cholesterol esters. A sharp increase in cholesterol ester levels, particularly in the aged APOE ε4 diet-enriched group, might be suggestive of abnormal acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT) activity and/or levels. Age exerts appreciable effects on the brain lipidome, especially with regard to polar lipid species. 2013 Journal Article http://hdl.handle.net/20.500.11937/44156 10.1016/j.neurobiolaging.2013.03.012 Elsevier Inc. restricted |
| spellingShingle | High cholesterol Mass spectrometry High fat Oxysterols APOE KI mice Glycerophospholipids Lim, Wei Ling Lam, Sin Man Shui, Guanghou Mondal, Alinda Ong, Daniel Duan, Xinrui Creegan, Rhona martins, Ian Sharman, Matthew Taddei, Kevin Verdile, Giuseppe Wenk, Markus Martins, Ralph Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice |
| title | Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice |
| title_full | Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice |
| title_fullStr | Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice |
| title_full_unstemmed | Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice |
| title_short | Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice |
| title_sort | effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein e ε3 and ε4 knock-in mice |
| topic | High cholesterol Mass spectrometry High fat Oxysterols APOE KI mice Glycerophospholipids |
| url | http://hdl.handle.net/20.500.11937/44156 |