Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis
We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia,...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Published: |
Elsevier
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/43704 |
| _version_ | 1848756780433145856 |
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| author | Tong, S. Lilliebridge, R. Holt, D. Coombs, Geoffrey Currie, B. Giffard, P. |
| author_facet | Tong, S. Lilliebridge, R. Holt, D. Coombs, Geoffrey Currie, B. Giffard, P. |
| author_sort | Tong, S. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. © 2010. |
| first_indexed | 2025-11-14T09:17:38Z |
| format | Journal Article |
| id | curtin-20.500.11937-43704 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:17:38Z |
| publishDate | 2010 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-437042017-09-13T13:37:33Z Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis Tong, S. Lilliebridge, R. Holt, D. Coombs, Geoffrey Currie, B. Giffard, P. We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. © 2010. 2010 Journal Article http://hdl.handle.net/20.500.11937/43704 10.1016/j.diagmicrobio.2010.03.015 Elsevier restricted |
| spellingShingle | Tong, S. Lilliebridge, R. Holt, D. Coombs, Geoffrey Currie, B. Giffard, P. Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis |
| title | Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis |
| title_full | Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis |
| title_fullStr | Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis |
| title_full_unstemmed | Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis |
| title_short | Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis |
| title_sort | rapid detection of h and r panton-valentine leukocidin isoforms in staphylococcus aureus by high-resolution melting analysis |
| url | http://hdl.handle.net/20.500.11937/43704 |