Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis
We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia,...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Published: |
Elsevier
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/43704 |