Emulating a crowded intracellular environment in vitro dramatically improves RT-PCR performance

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Emulating a crowded intracellular environment in vitro dramatically improves RT-PCR performance

The polymerase chain reaction’s (PCR) phenomenal success in advancing fields as diverse as Medicine, Agriculture, Conservation, or Paleontology is based on the ability of using isolated prokaryotic thermostable DNA polymerases in vitro to copy DNA irrespective of origin. This process occurs intracel...

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Bibliographic Details
Main Authors:	Lareu, Ricky R., Harve, K., Raghunath, M.
Format:	Journal Article
Published:	Academic Press 2007
Subjects:	Sensitivity Macromolecular crowding Reverse transcriptase Macromolecule Polymerase chain reaction DNA polymerase Excluded volume effect Reverse transcription
Online Access:	http://hdl.handle.net/20.500.11937/41966

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