Emulating a crowded intracellular environment in vitro dramatically improves RT-PCR performance

Emulating a crowded intracellular environment in vitro dramatically improves RT-PCR performance

The polymerase chain reaction’s (PCR) phenomenal success in advancing fields as diverse as Medicine, Agriculture, Conservation, or Paleontology is based on the ability of using isolated prokaryotic thermostable DNA polymerases in vitro to copy DNA irrespective of origin. This process occurs intracel...

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Bibliographic Details
Main Authors: Lareu, Ricky R., Harve, K., Raghunath, M.
Format: Journal Article
Published: Academic Press 2007
Subjects:
Sensitivity
Macromolecular crowding
Reverse transcriptase
Macromolecule
Polymerase chain reaction
DNA polymerase
Excluded volume effect
Reverse transcription
Online Access:http://hdl.handle.net/20.500.11937/41966

Internet

http://hdl.handle.net/20.500.11937/41966

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