Theoretical approaches to identify the potent scaffold for human sirtuin1 activator: Bayesian modeling and density functional theory

Bayesian and pharmacophore modeling approaches were utilized to identify the fragments and critical chemical features of small molecules that enhance sirtuin1 (SIRT1) activity. Initially, 48 Bayesian models (BMs) were developed by exploring 12 different fingerprints (ECFC, ECFP, EPFC, EPFP, FPFC, FP...

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Main Authors: Sakkiah, S., Arooj, Mahreen, Lee, K., Torres, J.
Format: Journal Article
Published: Birkhaeuser Science 2014
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/40389
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author Sakkiah, S.
Arooj, Mahreen
Lee, K.
Torres, J.
author_facet Sakkiah, S.
Arooj, Mahreen
Lee, K.
Torres, J.
author_sort Sakkiah, S.
building Curtin Institutional Repository
collection Online Access
description Bayesian and pharmacophore modeling approaches were utilized to identify the fragments and critical chemical features of small molecules that enhance sirtuin1 (SIRT1) activity. Initially, 48 Bayesian models (BMs) were developed by exploring 12 different fingerprints (ECFC, ECFP, EPFC, EPFP, FPFC, FPFP, FCFC, FCFP, LCFC, LCFP, LPFC, and LPLP) with diameters of 4, 6, 8, and 10. Among them the BM1 model was selected as the best model based on its good statistical parameters including total accuracy: 0.98 and positive recalls: 0.95. Additionally, BM1 showed good predictive power for the test set (total accuracy: 0.87 and positive recall: 0.87). In addition, 10 qualitative pharmacophore models were generated using 6 well-known SIRT1 activators. Hypothesis2 (Hypo2) was selected as best hypothesis, among 10 Hypos, based on its discriminant ability between the highly active and least/moderately active SIRT1 activators. The best models, BM1 and Hypo2 were used as a query in virtual screens of a drug-like database and the hit molecules were sorted based on Bayesian score and fit value, respectively. In addition, the highest occupied molecular orbital, lowest unoccupied molecular orbital, and energy gap values were calculated for the selected virtual screening hits using density functional theory. Finally, 16 compounds were selected as leads based on their energy gap values, which represent the high reactivity of molecules. Thus, our results indicated that the combination of two-dimensional (2D) and 3D approaches are useful for the discovery and development of specific and potent SIRT1 activators, and will benefit medicinal chemists focused on designing novel lead compounds that activate SIRT1.
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spelling curtin-20.500.11937-403892017-09-13T13:39:37Z Theoretical approaches to identify the potent scaffold for human sirtuin1 activator: Bayesian modeling and density functional theory Sakkiah, S. Arooj, Mahreen Lee, K. Torres, J. Sirtuin Density functional theory Ligand-based pharmacophore model Activator Bayesian model Bayesian and pharmacophore modeling approaches were utilized to identify the fragments and critical chemical features of small molecules that enhance sirtuin1 (SIRT1) activity. Initially, 48 Bayesian models (BMs) were developed by exploring 12 different fingerprints (ECFC, ECFP, EPFC, EPFP, FPFC, FPFP, FCFC, FCFP, LCFC, LCFP, LPFC, and LPLP) with diameters of 4, 6, 8, and 10. Among them the BM1 model was selected as the best model based on its good statistical parameters including total accuracy: 0.98 and positive recalls: 0.95. Additionally, BM1 showed good predictive power for the test set (total accuracy: 0.87 and positive recall: 0.87). In addition, 10 qualitative pharmacophore models were generated using 6 well-known SIRT1 activators. Hypothesis2 (Hypo2) was selected as best hypothesis, among 10 Hypos, based on its discriminant ability between the highly active and least/moderately active SIRT1 activators. The best models, BM1 and Hypo2 were used as a query in virtual screens of a drug-like database and the hit molecules were sorted based on Bayesian score and fit value, respectively. In addition, the highest occupied molecular orbital, lowest unoccupied molecular orbital, and energy gap values were calculated for the selected virtual screening hits using density functional theory. Finally, 16 compounds were selected as leads based on their energy gap values, which represent the high reactivity of molecules. Thus, our results indicated that the combination of two-dimensional (2D) and 3D approaches are useful for the discovery and development of specific and potent SIRT1 activators, and will benefit medicinal chemists focused on designing novel lead compounds that activate SIRT1. 2014 Journal Article http://hdl.handle.net/20.500.11937/40389 10.1007/s00044-014-0983-3 Birkhaeuser Science restricted
spellingShingle Sirtuin
Density functional theory
Ligand-based pharmacophore model
Activator
Bayesian model
Sakkiah, S.
Arooj, Mahreen
Lee, K.
Torres, J.
Theoretical approaches to identify the potent scaffold for human sirtuin1 activator: Bayesian modeling and density functional theory
title Theoretical approaches to identify the potent scaffold for human sirtuin1 activator: Bayesian modeling and density functional theory
title_full Theoretical approaches to identify the potent scaffold for human sirtuin1 activator: Bayesian modeling and density functional theory
title_fullStr Theoretical approaches to identify the potent scaffold for human sirtuin1 activator: Bayesian modeling and density functional theory
title_full_unstemmed Theoretical approaches to identify the potent scaffold for human sirtuin1 activator: Bayesian modeling and density functional theory
title_short Theoretical approaches to identify the potent scaffold for human sirtuin1 activator: Bayesian modeling and density functional theory
title_sort theoretical approaches to identify the potent scaffold for human sirtuin1 activator: bayesian modeling and density functional theory
topic Sirtuin
Density functional theory
Ligand-based pharmacophore model
Activator
Bayesian model
url http://hdl.handle.net/20.500.11937/40389