Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator
WWP1 is a ubiquitin ligase, associated with the post-translational regulation of several tumour-promoting and tumour suppressor proteins. Here we show that WWP1 expression is up-regulated in a subset of breast tumour cell lines and primary breast tumours. We overexpressed WWP1 in MCF10A breast epith...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
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John Wiley & Sons Ltd
2008
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| Online Access: | http://hdl.handle.net/20.500.11937/39791 |
| _version_ | 1848755688857141248 |
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| author | Huu, N. Ryder, W. Zeps, Nikolajs Flasza, M. Chiu, M. Hanby, A. Poulsom, R. Clarke, R. Baron, M. |
| author_facet | Huu, N. Ryder, W. Zeps, Nikolajs Flasza, M. Chiu, M. Hanby, A. Poulsom, R. Clarke, R. Baron, M. |
| author_sort | Huu, N. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | WWP1 is a ubiquitin ligase, associated with the post-translational regulation of several tumour-promoting and tumour suppressor proteins. Here we show that WWP1 expression is up-regulated in a subset of breast tumour cell lines and primary breast tumours. We overexpressed WWP1 in MCF10A breast epithelial cells and demonstrated increased cell growth and anchorage- independent colony formation. RNAi knockdown of WWP1 expression in T47D and MCF7 breast tumour cell lines reduced anchorage-independent colony formation. We used WWP1 protein expression levels, in combination with its subcellular localization, to classify breast tumours into four categories. Surprisingly, a category with low/absent WWP1 expression displayed a consistently worse prognosis compared with WWPl-expressing tumours. Importantly, the association with disease-free survival was independent of the status of other commonly used prognostic indicators. Thus, WWP1 is a prognostic marker and may be a potential therapeutic target for a subset of breast tumours. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. |
| first_indexed | 2025-11-14T09:00:17Z |
| format | Journal Article |
| id | curtin-20.500.11937-39791 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:00:17Z |
| publishDate | 2008 |
| publisher | John Wiley & Sons Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-397912017-09-13T14:30:53Z Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator Huu, N. Ryder, W. Zeps, Nikolajs Flasza, M. Chiu, M. Hanby, A. Poulsom, R. Clarke, R. Baron, M. WWP1 is a ubiquitin ligase, associated with the post-translational regulation of several tumour-promoting and tumour suppressor proteins. Here we show that WWP1 expression is up-regulated in a subset of breast tumour cell lines and primary breast tumours. We overexpressed WWP1 in MCF10A breast epithelial cells and demonstrated increased cell growth and anchorage- independent colony formation. RNAi knockdown of WWP1 expression in T47D and MCF7 breast tumour cell lines reduced anchorage-independent colony formation. We used WWP1 protein expression levels, in combination with its subcellular localization, to classify breast tumours into four categories. Surprisingly, a category with low/absent WWP1 expression displayed a consistently worse prognosis compared with WWPl-expressing tumours. Importantly, the association with disease-free survival was independent of the status of other commonly used prognostic indicators. Thus, WWP1 is a prognostic marker and may be a potential therapeutic target for a subset of breast tumours. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. 2008 Journal Article http://hdl.handle.net/20.500.11937/39791 10.1002/path.2385 John Wiley & Sons Ltd restricted |
| spellingShingle | Huu, N. Ryder, W. Zeps, Nikolajs Flasza, M. Chiu, M. Hanby, A. Poulsom, R. Clarke, R. Baron, M. Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator |
| title | Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator |
| title_full | Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator |
| title_fullStr | Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator |
| title_full_unstemmed | Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator |
| title_short | Tumour-promoting activity of altered WWPI expression in breast cancer and its utility as a prognostic indicator |
| title_sort | tumour-promoting activity of altered wwpi expression in breast cancer and its utility as a prognostic indicator |
| url | http://hdl.handle.net/20.500.11937/39791 |