The transformation of protoplasts of Leptosphaeria maculans to Hygromycin B resistance

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The transformation of protoplasts of Leptosphaeria maculans to Hygromycin B resistance

Conditions are described for the efficient isolation and regeneration of protoplasts of a fungal pathogen of brassicas, Leptosphaeria maculans. Treatment of the protoplasts with DNA of the plasmid pAN7-1 (containing an E. coli hygromycin phosphotransferase gene with Aspergillus nidulans expression s...

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Bibliographic Details
Main Authors:	Farman, M., Oliver, Richard
Format:	Journal Article
Published:	1988
Online Access:	http://hdl.handle.net/20.500.11937/39036

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