The transformation of protoplasts of Leptosphaeria maculans to Hygromycin B resistance
Conditions are described for the efficient isolation and regeneration of protoplasts of a fungal pathogen of brassicas, Leptosphaeria maculans. Treatment of the protoplasts with DNA of the plasmid pAN7-1 (containing an E. coli hygromycin phosphotransferase gene with Aspergillus nidulans expression s...
| Main Authors: | , |
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| Format: | Journal Article |
| Published: |
1988
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| Online Access: | http://hdl.handle.net/20.500.11937/39036 |