Characterization of Maleimide-Based Glycogen Synthase Kinase-3 (GSK-3) Inhibitors as Stimulators of Steroidogenesis

Inhibition of GSK-3β has been well documented to account for the behavioral actions of the mood stabilizer lithium in various animal models of mood disorders. Recent studies have showed that genetic or pharmacological inhibition of GSK-3β resulted in anxiolytic-like and pro-social behavior. In our o...

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Main Authors: Gunosewoyo, Hendra, Midzak, A., Gaisina, I., Sabath, E., Fedolak, A., Hanania, T., Brunner, D., Papadopoulos, V., Kozikowski, A.
Format: Journal Article
Published: American Chemical Society 2013
Online Access:http://hdl.handle.net/20.500.11937/3603
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author Gunosewoyo, Hendra
Midzak, A.
Gaisina, I.
Sabath, E.
Fedolak, A.
Hanania, T.
Brunner, D.
Papadopoulos, V.
Kozikowski, A.
author_facet Gunosewoyo, Hendra
Midzak, A.
Gaisina, I.
Sabath, E.
Fedolak, A.
Hanania, T.
Brunner, D.
Papadopoulos, V.
Kozikowski, A.
author_sort Gunosewoyo, Hendra
building Curtin Institutional Repository
collection Online Access
description Inhibition of GSK-3β has been well documented to account for the behavioral actions of the mood stabilizer lithium in various animal models of mood disorders. Recent studies have showed that genetic or pharmacological inhibition of GSK-3β resulted in anxiolytic-like and pro-social behavior. In our ongoing efforts to develop GSK-3β inhibitors for the treatment of mood disorders, SAR studies on maleimide-based compounds were undertaken. We present herein for the first time that some of these GSK-3β inhibitors, in particular analogues 1 and 9, were able to stimulate progesterone production in the MA-10 mouse tumor Leydig cell model of steroidogenesis without any significant toxicity. These two compounds were tested in the SmartCube behavioral assay and showed anxiolytic-like signatures following daily dose administration (50 mg/kg, ip) for 13 days. Taken together, these results support the hypothesis that GSK-3β inhibition could influence neuroactive steroid production thereby mediating the modulation of anxiety-like behavior in vivo.
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publishDate 2013
publisher American Chemical Society
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spelling curtin-20.500.11937-36032018-03-29T09:05:22Z Characterization of Maleimide-Based Glycogen Synthase Kinase-3 (GSK-3) Inhibitors as Stimulators of Steroidogenesis Gunosewoyo, Hendra Midzak, A. Gaisina, I. Sabath, E. Fedolak, A. Hanania, T. Brunner, D. Papadopoulos, V. Kozikowski, A. Inhibition of GSK-3β has been well documented to account for the behavioral actions of the mood stabilizer lithium in various animal models of mood disorders. Recent studies have showed that genetic or pharmacological inhibition of GSK-3β resulted in anxiolytic-like and pro-social behavior. In our ongoing efforts to develop GSK-3β inhibitors for the treatment of mood disorders, SAR studies on maleimide-based compounds were undertaken. We present herein for the first time that some of these GSK-3β inhibitors, in particular analogues 1 and 9, were able to stimulate progesterone production in the MA-10 mouse tumor Leydig cell model of steroidogenesis without any significant toxicity. These two compounds were tested in the SmartCube behavioral assay and showed anxiolytic-like signatures following daily dose administration (50 mg/kg, ip) for 13 days. Taken together, these results support the hypothesis that GSK-3β inhibition could influence neuroactive steroid production thereby mediating the modulation of anxiety-like behavior in vivo. 2013 Journal Article http://hdl.handle.net/20.500.11937/3603 10.1021/jm400511s American Chemical Society restricted
spellingShingle Gunosewoyo, Hendra
Midzak, A.
Gaisina, I.
Sabath, E.
Fedolak, A.
Hanania, T.
Brunner, D.
Papadopoulos, V.
Kozikowski, A.
Characterization of Maleimide-Based Glycogen Synthase Kinase-3 (GSK-3) Inhibitors as Stimulators of Steroidogenesis
title Characterization of Maleimide-Based Glycogen Synthase Kinase-3 (GSK-3) Inhibitors as Stimulators of Steroidogenesis
title_full Characterization of Maleimide-Based Glycogen Synthase Kinase-3 (GSK-3) Inhibitors as Stimulators of Steroidogenesis
title_fullStr Characterization of Maleimide-Based Glycogen Synthase Kinase-3 (GSK-3) Inhibitors as Stimulators of Steroidogenesis
title_full_unstemmed Characterization of Maleimide-Based Glycogen Synthase Kinase-3 (GSK-3) Inhibitors as Stimulators of Steroidogenesis
title_short Characterization of Maleimide-Based Glycogen Synthase Kinase-3 (GSK-3) Inhibitors as Stimulators of Steroidogenesis
title_sort characterization of maleimide-based glycogen synthase kinase-3 (gsk-3) inhibitors as stimulators of steroidogenesis
url http://hdl.handle.net/20.500.11937/3603